摘要
目的研究高功率脉冲微波(high power pulsed microwave,HPPM)对产生物膜单增李斯特菌(Listeria monocytogenes)的抑制作用。方法对不同脉冲时间HPPM处理下生物膜的形成时间进行了测定,采用光学显微镜、扫描电子显微镜和激光共聚焦显微镜对HPPM作用下生物膜的厚度、致密程度与黏附性进行了观察。结果不同脉冲时间HPPM处理在24 h内可延缓单增李斯特菌生物膜的形成。对照组单增李斯特菌孵育24 h后形成均匀致密的生物膜结构,与之相比,经过HPPM(200 Hz、9 min)处理后,菌体表现出更稀薄和松散的细胞聚集状态。对照组的生物膜覆盖了玻璃层的整个表面,HPPM处理组覆盖层的表面覆盖度和细菌密度显著下降。对照组形成的生物膜厚度为(51.67±2.32)μm,HPPM处理后生物膜厚度降至(19.88±1.79)μm。结论HPPM处理对生物膜厚度、致密程度与黏附性均有显著影响,能够有效抑制单增李斯特菌生物膜的形成,具有保障食品质量安全的开发潜力。
Objective To investigate the inhibitory effects of high power pulsed microwave(HPPM)on biofilm-forming of Listeria monocytogenes.Methods The formation time of biofilms under HPPM treatment with different pulse times were measured.Optical microscope,scanning electron microscope and confocal laser scanning microscope were used to observe the thickness,compactness and adhesiveness of biofilms after HPPM treatment.Results HPPM treatment with different pulse times delayed the formation time of Listeria monocytogenes biofilms within 24-hour incubation.A uniform and dense biofilm structure was formed in the control group after 24-hour incubation with Listeria monocytogenes.In comparison,a sparse and loose state of cell aggregation was exhibited after HPPM treatment(200 Hz,9 min).The biofilm in the control group covered the entire surface of the glass layer.The surface coverage and bacterial density of the covering layer were significantly decreased in the HPPM treatment group.The thickness of the biofilm formed in the control group was(51.67±2.32)μm,and it was decreased to(19.88±1.79)μm after HPPM treatment.Conclusion HPPM treatment has a significant impact on the thickness,compactness and adhesion of the Listeria monocytogenes biofilm.It effectively inhibits the formation of Listeria monocytogenes biofilm and is expected to become a potential technology to ensure the food quality and safety of products.
作者
王帆
冯洪燕
张亚新
张斌
刘小莉
WANG Fan;FENG Hong-Yan;ZHANG Ya-Xin;ZHANG Bin;LIU Xiao-Li(Institute of Farm Product Processing,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Lianyungang Comprehensive Inspection and Testing Center for Quality and Technology,Lianyungang 222006,China;School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,China;Changshu Jintangshi Aquatic Products Company Limited,Changshu 215542,China)
出处
《食品安全质量检测学报》
2025年第18期120-125,共6页
Journal of Food Safety and Quality
关键词
高功率脉冲微波
单增李斯特菌
生物膜
high power pulse microwave
Listeria monocytogenes
biofilm
