摘要
目的探讨异甘草素对NK/T细胞淋巴瘤细胞增殖、侵袭功能及免疫杀伤敏感性的影响。方法采用多浓度(0、2.5、5、12µmol/L)异甘草素在多时间点(0、12、24、48 h)干预后,检测NK/T细胞淋巴瘤细胞SNK-1和SNT-8的细胞活力。并在不同浓度的异甘草素干预后,采用克隆形成法、流式细胞术、Transwell细胞侵袭实验观察SNK-1和SNT-8细胞的增殖、凋亡及侵袭能力特征改变情况。提取分离NK细胞后,采用乳酸脱氢酶释放法检测异甘草素作用前后SNK-1细胞对NK细胞的杀伤敏感性改变。采用流式细胞术检测异甘草素作用前后SNK-1细胞表面NKG2D配体(MICA/B、ULBP1、ULBP2及ULBP3)和HLA-Ⅰ类分子的表达改变。结果异甘草素以剂量和时间依赖方式分别抑制NK/T细胞淋巴瘤细胞SNK-1和SNT-8的增殖(P均<0.05)。与未处理相比,经异甘草素处理后的SNK-1和SNT-8细胞的克隆形成及侵袭能力降低,而细胞凋亡率增加(P均<0.05)。在10∶1和20∶1的效靶比下,异甘草素均能使SNK-1细胞对NK细胞的杀伤敏感性增强(P均<0.05)。异甘草素作用后,SNK-1细胞表面的MICA/B、ULBP2和ULBP3的表达增加(P均<0.05)。结论异甘草素可抑制NK/T细胞淋巴瘤细胞的增殖和侵袭、促进其凋亡,并增加NK细胞对其免疫杀伤。
Objective To explore the effect of isoliquiritigenin on the proliferation,invasion and immune killing sensitivity of NK/T cell lymphoma.Methods After intervention with isoliquiritigenin at multiple concentrations(0,2.5,5 and 12µmol/L)and time points(0,12,24 and 48 h),the cell viability of NK/T cell lymphoma cells SNK-1 and SNT-8 was measured.Following intervention with different concentrations of isoliquiritigenin,clonogenic assays,flow cytometry and Transwell cell invasion experiments were used to detect changes in proliferation,apoptosis,and invasion capabilities of SNK-1 and SNT-8 cells.After extracting and isolating NK cells,the lactate dehydrogenase release assay was used to de‐tect the changes in the cytotoxicity sensitivity of SNK-1 cells to NK cells before and after the treatment of Isoliquiritigenin.Flow cytometry was used to detect the expression changes of NKG2D ligands(MICA/B,ULBP1,ULBP2 and ULBP3)and HLA class I molecules on the surface of SNK-1 cells before and after the treatment of isoliquiritigenin.Results Isoliquiritigenin inhibited the proliferation of NK/T cell lymphoma SNK-1 and SNT-8 in a dose-and time-dependent man‐ner,respectively(all P<0.05).Compared with the untreated group,SNK-1 and SNT-8 clone formation and cell invasion ability were weakened after treatment with isoliquiritigenin,and the apoptosis rate of cells increased(all P<0.05).At the effectual target ratios of 10∶1 and 20∶1,isoliquiritigenin could enhance the cytotoxicity sensitivity of SNK-1 cells to NK cells(all P<0.05).After the treatment of isoliquiritigenin,the expressions of MICA/B,ULBP2 and ULBP3 on the surface of SNK-1 cells was increased(all P<0.05).Conclusion Isoliquiritigenin inhibits the proliferation and invasion of NK/T cell lymphoma,promotes its apoptosis,and enhances NK cell-mediated immune killing against it.
作者
李晓燕
宋彩露
吴松
唐海林
黄国贤
LI Xiaoyan;SONG Cailu;WU Song;TANG Hailin;HUANG Guoxian(Department of Pharmacy,Sun Yat-Sen University Cancer Center,Guangzhou 510060,China;State Key Laboratory of Oncology in South China,Sun Yat-Sen University Cancer Center,Guangzhou 510060,China;Department of General Studies,Sun Yat-Sen University Cancer Center,Guangzhou 510060,China)
出处
《老年医学研究》
2025年第4期45-48,共4页
Geriatrics Research
基金
广东省自然科学基金资助项目(2022B1515120087,KLF5调控脂质重编程促进NK/T细胞淋巴瘤进展的机制研究)。
