摘要
以前期从土壤中分离的一株能够利用纤维素产油脂的少孢哈萨克斯坦酵母为出发菌株,对其进行室温常压等离子(ARTP)诱变。从诱变所得菌株中随机挑选30株,得正向突变株17株,负向突变株13株,正向突变率为56.67%。正向突变株中编号为m21的菌株为最优突变株,最高油脂产量达到(0.622±0.017)g/L,比出发菌株提高了1.49倍,并且诱变没有改变菌株的生长特性。经诱变选育所得菌株具有很好的工业应用前景。
A Kazachstania exigua strain which was isolated from soil and could produce lipid using cellulose,was mutated using atmospheric room temperature plasma(ARTP).30 strains were randomly selected from the mutated strains,resulting in 17 positive mutant strains and 13 negative mutant strains,with a positive mutation rate of 56.67%.The positive mutant strain numbered m21 was the optimal mutant strain,with a maximum lipid production of 0.622±0.017 g/L,which was 1.49 times higher than that of the original strain.Moreover,the mutation did not change the growth characteristics of the strain.The strain obtained by mutation breeding in this study has good prospects for industrial application.
作者
王来友
程爽
刘冰冰
吴鹏宇
俞亚东
WANG Lai-you;CHENG Shuang;LIU Bing-bing;WU Peng-yu;YU Ya-dong(School of Biological and Chemical Engineering,Nanyang Institute of Technology,Nanyang 473004,China;College of Biological and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 211816,China)
出处
《南阳理工学院学报》
2025年第4期91-95,共5页
Journal of Nanyang Institute of Technology
基金
河南省科技攻关项目(222102320046,222102110261)
河南省高等学校重点科研项目计划(22A180025)
河南省工业微生物资源与发酵技术重点实验室开放课题(HIMFT20200204,HIMFT20210206,HIMFT20200101和HIMFT20210103)。
