摘要
目的观察子午流注针法治疗失眠症的疗效及其对谷氨酸(Glu)/γ-氨基丁酸(GABA)-谷氨酰胺(Gln)代谢环路的调节作用。方法40只雄性SD大鼠随机分为对照组、模型组、纳甲组和纳子组,10只/组。模型组、纳甲组和纳子组接受对氯苯丙氨酸腹腔注射后建立失眠大鼠模型并通过戊巴比妥钠睡眠协同实验进行模型评价。造模成功后,纳甲组与纳子组予以子午流注针法治疗7 d。采用苏木精-伊红染色观察下丘脑病理组织,采用高效液相色谱(HPLC)-质谱法(MS)/MS测定下丘脑中神经递质Glu和GABA的含量,采用免疫组织化学法(IHC)检测下丘脑中GABAA受体(GABAAR)的表达。采用免疫印迹法检测下丘脑谷氨酸脱羧酶(GAD65/67)和谷氨酰胺合成酶(GS)的表达水平。结果与模型组比较,子午流注针刺组谷氨酸含量降低(P<0.01),GABAA受体表达降低(P<0.01),GABA含量升高(P<0.05),谷氨酸/氨基丁酸比值降低。与模型组比较,子午流注针刺组下丘脑GAD65(P<0.01)、GAD67(P<0.05)蛋白表达增加,GS蛋白表达减少(P<0.01)。结论子午流注针刺可能通过调节Glu/GABAGln代谢,产生镇静催眠作用,影响Glu和GABA的合成和分解,恢复Glu和GABA之间的兴奋/抑制平衡。
Objective To investigate the therapeutic effect of Ziwuliuzhu acupuncture in a rat model of insomnia and its regulatory effect on the glutamic acid(Glu)/γ-aminobutyric acid(GABA)-glutamine(Gln)metabolic loop.Methods Forty male SD rats were randomly assigned to control group,model group,Najia group and Nazi group(n=10).In the latter 3 groups,rat models of insomnia were established by intraperitoneal injections of p-chlorophenylalanine and verified using a sodium pentobarbital-induced sleep test.After modeling,the rats in Najia and Nazi groups received acupuncture for 7 days at specifically chosen sets of acupoints based on the Ziwuliuzhu rationale in traditional Chinese medicine.Pathological changes in the hypothalamic tissue of the rats were examined with HE staining,and the levels of Glu and GABA in the hypothalamus were determined with high-performance liquid chromatography(HPLC)-mass spectrometry(MS)/MS.Immunohistochemistry was used to detect the expressions of GABAA receptors(GABAARs)in the hypothalamus,and the expression levels of glutamate decarboxylase(GAD65/67)and glutamine synthetase(GS)were determined with Western blotting.Results Compared with the model group,the rats in Najia and Nazi groups exhibited decreased Glu levels and GABAA receptor expression and increased GABA levels with a decreased Glu/GABA ratio in the hypothalamus.Ziwuliuzhu acupuncture significantly increased the protein expressions of GAD65 and GAD67 and lowered the expression of GS in the hypothalamus in the rat models of insomnia.Conclusion Ziwuliuzhu acupuncture produces sedative and hypnotic effects in rat models of insomnia possibly by regulating Glu and GABA-Gln metabolism to restore the excitatory/inhibitory balance between Glu and GABA.
作者
许嘉蓉
黄傲
丁祉恺
鲍宇
赵仓焕
蔡文智
XU Jiarong;HUANG Ao;DING Zhikai;BAO Yu;ZHAO Canghuan;CAI Wenzhi(Department of Nursing,Shenzhen Hospital,Southern Medical University,Shenzhen 518101,China;School of Nursing,Southern Medical University,Guangzhou 510515,China;Department of Acupuncture,First Affiliated Hospital of Jinan University,Guangzhou 510630,China)
出处
《南方医科大学学报》
北大核心
2025年第8期1616-1624,共9页
Journal of Southern Medical University
基金
国家自然科学基金(81973942)
深圳市医疗卫生三名工程(SZSM202211044)。
