摘要
目的:探讨微小核糖核酸-210-3p(microRNA-210-3p,miR-210-3p)在肺癌迁移和侵袭中的作用及分子机制。方法:通过裸鼠移植瘤模型检测miR-210-3p对肺癌荷瘤小鼠移植瘤生长及小鼠肺转移瘤形成的影响。通过RT-qPCR验证数据库分析所获得的靶基因表达水平;双荧光素酶报告基因实验检测miR-210-3p与下游靶基因BTG2的结合情况;采用平板克隆形成实验、CCK-8、Transwell实验分别检测BTG2表达变化对肺癌细胞增殖、迁移及侵袭能力的影响;Western Blot实验检测肺癌细胞转移相关分子蛋白表达变化。结果:miR-210-3p在肺癌组织中的表达水平高于癌旁组织;miR-210-3p过表达促进肺癌荷瘤小鼠移植瘤生长(P<0.001);miR-210-3p过表达组的小鼠肺内转移灶数量明显高于对照组(P<0.001);双荧光素酶基因报告实验显示,miR-210-3p可直接靶向结合BTG2;下调BTG2表达,肺癌细胞增殖、迁移及侵袭能力增强;上调BTG2表达,肺癌细胞增殖、迁移及侵袭能力减弱。结论:miR-210-3p可靶向调控BTG2表达,进而促进肺癌细胞增殖、迁移及侵袭。
Objective:To explore the effect and molecular mechanism of microRNA-210-3p(miR-210-3p)in lung cancer migration and invasion.Methods:The effects of miR-210-3p on the growth of transplanted tumors in mice with lung cancer and the development of lung metastases were assessed through animal experiments.RT-qPCR was employed to examine the target genes levels in databases.Dual luciferase reporter gene assay was used to detect the regulation of miR-210-3p on the downstream target gene BTG2.The plate cloning assay,CCK-8 and Transwell assay were employed to assess the effects of BTG2 expression on the proliferation,migration,and invasion capabilities of the lung cancer cells.Western blot assay was utilized to monitor changes in the protein expression levels of metastasis-related molecules within the lung cancer cells.Results:miR-210-3p was expressed at higher levels in lung cancer tissues than in paracancerous tissues.miR-210-3p overexpression promotes the growth of transplant tumor in mice with lung cancer(P<0.001),and the number of lung metastases in the mice in miR-210-3p overexpression group was significantly higher than control group(P<0.001).The dual-luciferase gene reporter assay demonstrated that miR-210-3p had the ability to directly target BTG2.Down-regulation of BTG2 expression in lung cancer cells enhanced their ability of proliferation,migration and invasion.Up-regulation of BTG2 expression weakened the ability of lung cancer cells to proliferation,migration and invasion.Conclusion:miR-210-3p can target and regulate BTG2 expression to promote the proliferation,migration and invasion of lung cancer cells.
作者
何君
陈莉
高琴
易光明
王丽斌
唐友攀
王力
HE Jun;CHEN Li;GAO Qin;YI Guangming;WANG Libin;TANG Youpan;WANG Li(Department of Oncology,the Third Hospital of Mianyang(Sichuan Mental Health Center),Sichuan Mianyang 621000,China;Department of Oncology,the Affiliated Hospital of Southwest Medical University,Sichuan Luzhou 646000,China;Department of Oncology,Shexian Hospital,Hebei Shexian 056400,China;Department of Gastroenterology,the People's Hospital of Zhongjiang,Sichuan Deyang 618100,China;Lung Cancer Center,West China Hospital of Sichuan University,Sichuan Chengdu 611135,China)
出处
《现代肿瘤医学》
2025年第10期1649-1657,共9页
Journal of Modern Oncology
基金
四川省卫生健康委员会医学科技项目(编号:21PJ006)
四川省抗癌协会临床科研(齐鲁)(编号:XH2022-101)。
