摘要
以马铃薯健康株根区土壤(HS)和疮痂病株根区土壤(OS)为研究对象,利用高通量测序技术分析了两组样品中微生物的多样性差异。结果表明,OS细菌群落的ACE指数、Chao1指数、香农(Shannon)指数均显著(p<0.05)高于HS,但HS和OS的真菌群落Alpha多样性指数无显著差异。与OS相比:在门水平上,HS变形菌门(Proteobacteria)、芽单胞菌门(Gemmatimonadota)、拟杆菌门(Bacteroidota)、担子菌门(Basidiomycota)的相对丰度降低,而酸杆菌门(Acidobacteriota)、放线菌门(Actinobacteriota)、绿弯菌门(Chloroflexi)、子囊菌门(Ascomycota)、壶菌门(Chytridiomycota)的相对丰度增加;在属水平上,HS鞘氨醇单胞菌属(Sphingomonas)、溶杆菌属(Lysobacter)、Tausonia腐质霉属(Humicola)、链格孢属(Alternaria)、被孢霉属(Mortierella)的相对丰度降低,而Sonoraphlyctis、青霉属(Penicillium)的相对丰度增加。主坐标分析(PcoA)及LEfSe分析的结果均表明,HS与OS的土壤微生物群落结构差异明显。在土壤理化性状上,HS的土壤pH值、速效钾含量显著低于OS,但其土壤有机质、碱解氮、有效磷含量均显著高于OS。功能预测结果显示,HS与OS在细菌的碳代谢和嘌呤代谢功能上存在显著差异。HS和OS的真菌群落以腐生营养型真菌为主,但OS木质腐生真菌的相对丰度显著高于HS。该研究初步揭示了马铃薯疮痂病株与健康株根区土壤的微生物结构与功能差异,可为马铃薯疮痂病的有效防控提供部分理论依据。
To explore the differences in soil microbial diversity,soil samples were collected from the root zone of healthy potato plants(HS)and scab-diseased potato plants(OS),and high-throughput sequencing was used.The results indicated that the ACE index,Chao1 index and Shannon index of the bacterial community in OS were significantly(p<0.05)higher than those of HS,yet there was no significant difference in the alpha diversity indexes for the fungal community.At the phylum level,compared with OS,the relative abundance of Proteobacteria,Gemmatimonadota,Bacteroidota and Basidiomycota in HS was decreased significantly,while the relative abundances of Acidobacteriota,Actinobacteriota,Chloroflexi,Ascomycota and Chytridiomycota in HS was increased significantly.At the genus level,the relative abundance of Sphingomonas,Lysobacter,Tausonia,Humicola,Alternaria and Mortierella dcreased in the HS,while the relative abundances of Sonoraphlyctis and Penicillium increased.Principal co-ordinates analysis(PcoA)and LEfSe analysis indicated that there was significant difference in the soil microbial community structure between HS and OS.For soil chemical properties,the pH value and available potassium content of HS was significantly lower than that of OS,yet the content of soil organic matter,alkali-hydrolyzable nitrogen,available phosphorus in HS was significantly higher.Functional prediction results showed that there were significant differences in bacterial carbon metabolism and purine metabolism functions.The fungal community was mainly saprotroph in both HS and OS,yet the relative abundance of wood saprotroph in the OS was significantly higher than that of HS.These findings revealed the differences in the structure and function of soil microorganisms in the root zone between scab-diseased potato plants and healthy plants,which could provide theoretical basis for the effective prevention and control of potato scab disease.
作者
谭海霞
彭红丽
王连龙
魏建梅
TAN Haixia;PENG Hongli;WANG Lianlong;WEI Jianmei(Department of Ecology,Hebei University of Environmental Engineering,Qinhuangdao 066102,Hebei,China;Hebei Key Laboratory of Agroecological Safety,Qinhuangdao 066102,Hebei,China)
出处
《浙江农业学报》
北大核心
2025年第8期1743-1754,共12页
Acta Agriculturae Zhejiangensis
基金
河北省重点研发计划(22322903D)
秦皇岛市科学技术研究与发展计划(202302B012)
河北省农业生态安全重点实验室开放基金(2023SYSJJ14)
河北环境工程学院基金项目(2023XJKT07)。
关键词
马铃薯疮痂病
根区土壤
微生物组成与结构
功能预测
potato common scab
root zone soil
microbial composition and structure
functional prediction
