摘要
目的:建立炒玉竹中果糖含量测定的分析方法。方法:样品用水加热回流提取,采用超高效液相色谱-电雾式检测器法测定,色谱条件:色谱柱为Agilent InfinityLab Poroshell 120 HILIC-Z(2.1 mm×100 mm,2.7μm),流动相为乙腈-0.05%甲酸铵溶液(梯度洗脱),流速为0.45 mL/min,柱温为30℃,进样量为2μL,CAD检测器参数:蒸发温度50℃、幂函数值1.00、数据采集率20 Hz、过滤常数3.6 s,外标法定量。结果:果糖的线性范围为0.1560~2.8079μg(r=0.9999),线性关系良好;重复性及稳定性的RSD均小于2.0%;加样回收率的范围在97.6%~102.7%,RSD为1.9%。10批炒玉竹样品的果糖含量为2.33%~3.67%。结论:所用方法灵敏度、准确度较高、重复性良好,操作简单便捷,可用于炒玉竹中果糖含量的测定。
Objective:To establish an analytical method for determining the content of fructose in Polygonati Odorati Rhizoma Tostum.Methods:The sample was heated and refluxed with water for extraction.UPLC-CAD was used for determination.The chromatographic conditions were as follows:the chromatographic column was Agilent InfinityLab Poroshell 120 HILIC-Z(2.1 mm×100 mm,2.7μm),the mobile phase was acetonitrile-0.05%ammonium formate solution(gradient elution),the flow rate was 0.45 ml min-1,the column temperature was 30℃,the sample volume was 2μL,and the CAD detector detects parameters were as follows:evaporation temperature 50℃,power function value 1.00,data collection rate 20 Hz,filter constant 3.6 s.Quantification was performed using the external standard method.Results:The linear range of fructose was 0.1560~2.8079μg(r=0.9999),with a good linear relationship;The RSD of repeatability and stability are both less than 2.0%;The recovery rate of fructose ranged from 97.6%to 102.7%,with an RSD of 1.9%.The fructose content of 10 batches of Polygonati Odorati Rhizoma Tostum samples was 2.33%~3.67%.Conclusion:The method used has high sensitivity,accuracy,good repeatability,simple and convenient operation,and can be used for the determination of fructose content in Polygonati Odorati Rhizoma Tostum.
作者
罗丽娟
周劲松
马恩耀
黄丹虹
林苗音
LUO Lijuan;ZHOU Jinsong;MA Enyao;HUANG Danhong;LIN Miaoyin(Guangdong Hanchao Traditional Chinese Medicine Technology Co.,Ltd./Guangdong Traditional Chinese Medicine Innovation Center,Guangzhou Guangdong 510000,China;Guangzhou Caizhilin Pharmaceutical Co.,Ltd.,Guangzhou Guangdong 510360,China;Guangyao Caizhilin〈Meizhou〉Pharmaceutical Co.,Ltd.,Meizhou Guangdong 514000,China)
出处
《江西化工》
2025年第4期33-36,44,共5页
Jiangxi Chemical Industry
基金
国家重点研发计划“中药饮片炒炙智能识别与生产控制技术研究”(2023YFC3504200)
2023年荔湾区产业领军人才(团队)扶持资金项目“广东省传统中药创新中心省级平台建设项目”(20250203)。
