摘要
蜂桶百花蜜产自四川达州万源富硒地区——“蜂桶中华蜜蜂(Apis cerana Fabr.)”遗传资源保护区,为国家农产品地理标志产品,但其风味特点、化学组分及生物活性尚不明确。论文采用顶空固相微萃取-气质联用鉴定到34种物质,以月桂醛、癸醛、2-十一酮、2-壬酮、苯乙醛、1-壬醇为关键风味物质,呈甜香、花香,而高海拔样本有玫瑰香气;硒含量22μg/kg;总酚及总黄酮分别为0.63~0.72 mg GAE/g与0.26~0.31 mg RE/g;采用超高效液相色谱-电喷雾-时间飞行串联质谱,结合非靶向代谢组学分析,鉴定到95种化合物,其中内南五味子酯A、脱氧基新隐丹参酮、鞣花酸、3-硫酸基槲皮素和异硬脂酸为所有样本共有非糖化合物,橙皮油内酯和Ostruthin为高+海拔样本特有,2,3-二羟基臭灵丹酸为低海拔样本特有。蜂桶百花蜜的DPPH、ABTS自由基半数清除浓度与铁离子还原力约为0.79~0.82 g/mL、0.27~0.30 g/mL,以及2.84~3.75 mmol/100 g。上述研究表明,蜂桶百花蜜为含硒中蜂蜜,且黄酮、酚酸、萜类含量丰富,抗氧化活性高,为“蜂桶蜂蜜”产品的开发及质量标准建立提供了数据支持。
Fengtong multifloral honey is produced in the“Fengtong Apis cerana Fabr.Genetic Resource Conservation Area,”located in the selenium-rich region of Wanyuan,Dazhou,Sichuan province.It is a certified product under the Geographical Indication system of China for agricultural products.In this work,the flavor characteristics,chemical composition,and bioactivities of Fengtong multifloral honey were investigated.A total of 34 compounds were detected using headspace solid-phase microextraction gas chromatography-mass spectrometry(HS-SPME-GC-MS).The key flavor substances of Fengtong multifloral honey are dodecanal,decanal,2-undecanone,2-nonanone,phenyl-acetaldehyde,and 1-nonanol,giving the honey sweet and floral flavors.In particular,high-altitude samples also possess a rose aroma.The selenium content of Fengtong multifloral honey is 22μg/kg,and the total phenolic content ranges from 0.63 to 0.72 mg GAE/g,while the total flavonoid content is 0.26~0.31 mg RE/g.A total of 95 compounds were identified using ultra-performance liquid chromatography-electrospray ionization time-of-flight tandem mass spectrometry(UPLC-ESI-TOF-MS/MS)combined with widely targeted metabolomics.The non-sugar compounds commonly detected in all samples were interiotherins A,deoxyneocryptotanshinone,ellagic acid,quercetin 3-sulfate,and isostearic acid.Auraptene and ostruthin were only identified in high-altitude samples,while 2α,3β-dihydroxdeoxypterodontic acid was found in low-altitude samples.Remarkable antioxidant activities were noted in all Fengtong multifloral honey samples,with IC50 values for DPPH and ABTS+radical scavenging and a ferric reducing antioxidant power of 0.79~0.82 g/mL,0.27~0.30 g/mL,and 2.84~3.75 mmol/100 g,respectively.In summary,Fengtong multifloral honey is selenium-containing honey produced by Apis cerana Fabr.with abundant flavonoids,phenolic acids,and terpenes,and remarkable antioxidant activities.These findings offer data support for the development of Fengtong honey products and their quality control standards.
作者
莫晓慧
杨晨
罗娅
刘芯宇
刘怡君
周新雨
陈逸菲
杨毅
段飞霞
MO Xiaohui;YANG Chen;LUO Ya;LIU Xinyu;LIU Yijun;ZHOU Xinyu;CHEN Yifei;YANG Yi;DUAN Feixia(College of Biomass Science and Engineering,Key Laboratory of Food Science and Technology in Sichuan Province,Sichuan University,Chengdu 610056,China;Wanyuan Mizhiyuan Agricultural Technology Development Co.Ltd.,Dazhou 636350,China)
出处
《现代食品科技》
北大核心
2025年第7期325-340,共16页
Modern Food Science and Technology
基金
四川大学-达州市人民政府校市战略合作专项(2021CDDZ-17)
国家自然科学基金(32102109)。
关键词
百花蜜
挥发性成分
功能组分
气质联用
液质联用
抗氧化
multifloral honey
volatiles
functional components
GC-MS
UPLC-ESI-TOF-MS/MS
antioxidants
