摘要
目的:建立肉桂叶的HPLC指纹图谱,并结合化学计量学和一测多评法评价不同批次肉桂叶的质量。方法:采用HPLC法建立了12批肉桂叶的指纹图谱并进行相似度评价,采用聚类分析(CA)和主成分分析(PCA),对不同批次肉桂叶进行化学模式识别分析。以肉桂酸为内标参照物,建立一测多评(QAMS)法,计算2-羟基肉桂醛、肉桂醇、桂皮醛、邻甲氧基肉桂醛的相对校正因子,采用外标(ESM)法和QASM法测定肉桂叶中2-羟基肉桂醛、肉桂醇、肉桂酸、桂皮醛、邻甲氧基肉桂醛的含量。结果:指纹图谱共确定12个共有峰,通过对照品指认了7个化学成分,除S3与S11批次外,其余批次肉桂叶样品与对照指纹图谱的相似度均>0.9;CA将12批肉桂叶分为2类;PCA显示不同批次间肉桂叶的化学成分存在一定的差异,并确定了3个主成分,累计方差贡献率为86.022%;QAMS与ESM测定的以上5个化合物含量值无明显差异。结论:建立的肉桂叶指纹图谱及含量测定方法稳定、可靠,可为肉桂叶的质量控制及评价提供参考。
Objective:To establish the HPLC fingerprint and evaluate the quality of different batches of Folium Cinnamoni by chemometrics and quantitative analysis of multi-components with a single-marker(QAMS).Methods:HPLC was used to establish the fingerprints of 12 batches of Folium Cinnamoni and perform similarity evaluation.And then principal component analysis(PCA)and cluster analysis(CA)were used to analyze the chemical pattern of different batches of Folium Cinnamoni.The QAMS method was established by using cinnamic acid as the internal standard reference.The relative correction factors of 2-hydroxy cinnamaldehyde,cinnamyl alcohol,trans-cinnamaldehyde and 2'-methoxy cinnamaldehyde were calculated.The contents of 2-hydroxycinnamaldehyde,cinnamyl alcohol,cinnamic acid,trans-cinnamaldehyde and 2'-methoxy cinnamaldehyde in Folium Cinnamoni were determined by external standard(ESM)method and QAMS method.Results:A total of 12 common peaks were identified from the fingerprint,and 7 of them were confirmed by reference substance.CA divided 12 batches of Folium Cinnamoni into 2 categories;PCA showed that there were some differences in the chemical composition of them.Three principal components were identified,and the result showed that the cumulative variance contribution rate was 86.022%.The contents of these 5 compounds measured by QAMS and ESM showed no significant difference.Conclusion:The fingerprint and content determination method of Folium Cinnamoni are stable and reliable,which can provide reference for quality control and evaluation of Folium Cinnamoni.
作者
丁富娟
张静
苑艳飞
王涛
刘玉玲
纪国力
DING Fu-juan;ZHANG Jing;YUAN Yan-fei;WANG Tao;LIU Yu-ling;JI Guo-li(Taian Institute for Food and Drug Control,Taian Fiber Inspection Institute,Taian 271000,China)
出处
《药物分析杂志》
北大核心
2025年第6期996-1004,共9页
Chinese Journal of Pharmaceutical Analysis
基金
2024国家药品标准物质协作研究与标定2024-W0203004。
关键词
肉桂叶
指纹图谱
化学计量学
含量测定
质量控制
Folium Cinnamoni
fingerprints
stoichiometry
content determination
quality control
