摘要
目的:研究不同干燥方式对大红袍果皮香气成分的影响及差异情况。方法:采用顶空固相微萃取结合气相色谱-质谱联用技术(headspace solid phase microextraction and gas chromatography-mass spectrometry,HSSPME-GC-MS)分析5种干燥方式(热风干燥、真空干燥、室内恒温干燥、微波干燥和自然晾晒)处理后大红袍果皮中香气成分的种类和相对含量,经正交偏最小二乘判别分析(OPLS-DA),筛选出变量重要性投影(VIP)值≥1的差异香气成分。结果:大红袍果皮中共检出香气成分94个,其中烯烃类20个、酯类20个,醇类16个,醛类9个,酸类7个,酮类5个,酚类3个,以及其他种类(包括醚类、芳香类等)14个,有36个共有成分,58个特异性成分,含量最高的是D-柠檬烯(41.30%±1.45%)。所建立OPLS-DA模型稳定可靠,通过VIP法筛选出28个差异香气成分,通过相对气味活度值(ROAV)分析,确定了4个关键香气成分,分别为D-柠檬烯、芳樟醇、二氢猕猴桃内酯和反式橙花叔醇,其中,D-柠檬烯香气贡献最大。结论:本研究利用HS-SPME-GC-MS技术分析5种干燥方式下大红袍果皮香气成分,OPLS-DA模型和聚类分析能较好地区分不同干燥方式的大红袍果皮。筛选出来28个差异香气成分及4个关键香气成分。其中,采用微波干燥的大红袍果皮关键香气成分相对含量最高,热风干燥次之。本研究为大红袍果皮干燥方式选择及药食同源资源开发提供参考。
Objective:In order to study the effects of different drying methods on the aroma components of Citrus reticulata'Dahongpao'Peel and their differences.Methods:Headspace solid phase microextraction combined with gas chromatography-mass spectrometry(HS-SPME-GC-MS)was used to analyze the types and relative contents of aroma components in Citrus reticulata'Dahongpao'Peel after five drying methods(including hot air drying,vacuum drying,indoor constant temperature drying,microwave drying and natural drying).The orthogonal partial least squares-discriminant analysis(OPLS-DA)was used to screen differential volatile compounds with a variable importance in projection(VIP)value≥1.Results:The results showed that a total of 94 aroma components were detected,including 20 olefins,20 esters,16 alcohols,9 aldehydes,7 acids,5 ketones,3 phenols,14 other compounds(including ethers,aromatics,etc.).Among them,36 common components and 58 specific components,and the highest content was D-limonene(41.30%±0.85%).The established OPLS-DA model is stable and reliable,and 28 different aroma components were screened by VIP method.Through the relative odor activity value(ROAV)determined four key aroma compounds including D-limonene,linalool,dihydroactinidiolide,and trans-nerolidol,with D-limonene contributing most significantly to the aroma profile.Conclusion:This study analyzed the volatile compounds in Citrus reticulata'Dahongpao'Peel under five drying methods using HS-SPME-CC-MS.The OPLS-DA model and cluster analysis successfully screened 28 differential volatile compounds and identified 4 key aroma compounds,effectively distinguishing Citrus reticulata'Dahongpao'Peel processed by different drying methods.Among the methods,microwave drying yielded peels with the highest relative content of key aroma compounds,followed by hot air drying.These findings provide valuable references for selecting drying methods for Citrus reticulata'Dahongpao'Peel and developing its potential as a medicinal and food resource.
作者
黄文平
牟腊梅
黄诗婷
郭冬琴
周文钊
张华
蒋忠岑
熊双
万莉
颜嘉利
HUANG Wen-ping;MOU La-mei;HUANG Shi-ting;GUO Dong-qin;ZHOU Wen-zhao;ZHANG Hua;JIANG Zhong-cen;XIONG Shuang;WAN Li;YAN Jia-li(College of Biology and Food engineering,Chongqing Three Gorges University;Chongqing Key Laborattory of Development and Utilization of Genuine Medicinal in Three Gorges Reservoir Area;Chongqing Institute for Food and Drug Control;School of Pharmacy,Chongqing Three Gorges Medical College;Green Planting and Deep Processing for the Three Gorges Reservoir Region Indigenous Medicinal Herbs of Chongqing Engineering Research Center)
出处
《药物分析杂志》
北大核心
2025年第6期947-959,共13页
Chinese Journal of Pharmaceutical Analysis
基金
重庆市教育委员会科学技术研究项目(KJZD-M202301204)
重庆市三峡库区道地药材开发与利用重点实验室项目(Sys20210029)
重庆三峡医药高等专科学校高层次人才科研启动基金项目(06020605029)。
关键词
大红袍果皮
干燥方式
固相微萃取-气相色谱-质谱联用技术
香气成分
正交偏最小二乘判别分析
相对气味活度
Citrus reticulata'Dahongpao'Peel
different drying methods
headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry
aroma components
orthogonal partial least squares discriminant analysis
relative odor activity
