摘要
The resistance and immune evasion of methicillin-resistant Staphylococcus aureus(MRSA)in biofilms are the culprits behind persistent infections.There is an urgent need for safe and effective antibacterial strategies to address MRSA and biofilm-related infections.Herein,we propose the development of an allin-one optical microfiber that integrates rapid quantitative analysis with synergistic antimicrobial therapy for deep-seated MRSA in biofilms.The prepared interfacial-functionalized sensor can be used for quantitative analysis of MRSA in clinical whole-blood samples with low volumes(10μL),reducing the detection time to 30 min and effectively preventing false-positive and false-negative results.The sensor can also be used for multimode antimicrobial therapy.This one-time treatment accelerates recovery and prevents recurrence through the synergistic effect of photothermal therapy,photodynamic therapy,and the antibacterial effect of Ag^(+),as well as the activation of immune memory.The therapy is localized with relatively low hyperthermia and does not cause harm to the surrounding healthy tissues.The integration of therapeutic agents onto the optical microfiber precludes their enrichment in other organs.The light guided through the optical fiber can reach deep-seated biofilms,which other light sources fail to reach.This work is promising for the clinical diagnosis and treatment of deep-seated infections.
基金
supported by the National Natural Science Foundation of China(No.62375108)
the Natural Science Foundation of Guangdong Province(No.2024A1515010088)
the Guangzhou Science and Technology Plan Project(No.2023A03J0130)
the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(No.2019BT02X105)
the Science and Technology Project in Guangzhou(No.2024A03J0812)
the Postdoctoral Fellowship Program(Grade B)of China Postdoctoral Science Foundation(No.GZB20230265).
