摘要
In the current study,the Zygomycetes fungus Cunninghamella elegans NRRL Y-1392 was evaluated for its ability to grow in extracts derived from dried and ground agricultural residues,such as mushroom stalks and roots from hydroponically cultivated lettuces and produce poly-unsaturated fatty acids(PUFA)andγ-linolenic acid(GLA)rich lipids.Initially,the compositions of stalks and lettuce roots were analysed,and the fungus was batch-flask cultivated on six different commercial semi-defined substrates containing different sugars detected in stalks and roots to evaluate its catabolic ability.C.elegans was capable to assimilate all sugars,but at a lower rate in the case of arabinose.Subsequently,C.elegans was cultivated on tailor-made semi-defined commercial substrates,resembling hydrolysates containing carbohydrates found in mushroom stalks,under both nitrogen-excess and nitrogen-limited conditions,and resembling that of hydrolysates of roots,under nitrogen-excess conditions.Based on the results,under nitrogen-excess conditions,in the case of media resembling stalks hydrolysates,higher production values for biomass,PUFAs,and GLA were observed(20.3 g/L,1906 mg/L,668 mg/L),accompanied by high productivity values due to short cultivation periods,while under nitrogen limitation,high lipid accumulation(lipid in dry cell weight=48%,w/w)was presented,and lipids rich in oleic acid were produced.Finally,the fungus was cultivated on a medium derived from hot water-extraction applied to mush-room stalks,enriched with organic nitrogen sources.The fungus was successfully grown on the sugar-rich waterextract derived from mushroom stalks,resulting in dry biomass of 14.5 g/L,lipids of 1.8 g/L,with 15%(w/w)of GLA in cellular lipids.
基金
funded within the framework of the Project Operational Program“Research and Innovation synergies in the Attica region”,project code:ATTP4-0339570,MIS 5185063,acronym“Residues2value”by the Hellenic Republic and the European Union.
