摘要
ABCG5是参与植物生长发育的转运蛋白。为研究ABCG5基因的功能,以浙江红花油茶为材料,利用RT-PCR技术克隆浙江红花油茶CchABCG5基因的编码序列(CDS),通过生物信息学分析、亚细胞定位和拟南芥遗传转化,对该基因功能进行初步分析。结果表明,CchABCG5基因CDS长度为1935 bp,编码644个氨基酸,有1个核苷酸结合结构域和1个跨膜结构域,是WBC型半分子转运蛋白。亚细胞定位分析显示,CchABCG5蛋白定位在细胞膜上。系统发育分析表明,CchABCG5蛋白与茶树的亲缘关系最近。CchABCG5过表达拟南芥表现出莲座叶减少和早花表型,相关开花基因表达量显著升高,种子和叶片的脂质含量升高。因此,推测CchABCG5基因在浙江红花油茶开花调控和脂质转运中发挥作用。
ABCG5 is a transporter involved in plant growth and development.To investigate the function of the ABCG5 gene,Camellia chekiangoleosa Hu was used as experimental material.The coding sequence(CDS)of the CchABCG5 gene was obtained by RT-PCR amplification,and the gene function was preliminarily analyzed by bioinformatics analysis,subcellular localization and genetic transformation of Arabidopsis thaliana.The results showed that the CDS length of CchABCG5 gene was 1935 bp,encoding 644 amino acids,with one nucleotide binding domain and one transmembrane domain,and it was a WBC-type half-sized transporter.Subcellular localization analysis showed that CchABCG5 protein was localized in the cell membrane,and phylogenetic analysis indicated that the CchABCG5 protein shares the closest evolutionary relationship with that of tea plant(Camellia sinensis).CchABCG5-overexpression Arabidopsis thaliana exhibited a reduced number of rosette leaves,an early flowering phenotype,and significantly elevated expression levels of flowering-related genes,as well as increased lipid content in seeds and leaves.Therefore,it was speculated that CchABCG5 gene played a role in flowering regulation and lipid transport in Camellia chekiangoleosa Hu.
作者
缪百灵
陈娟娟
李亮杰
楚宗丽
董向向
MIAO Bailing;CHEN Juanjuan;LI Liangjie;CHU Zongli;DONG Xiangxiang(College of Horticulture,Xinyang Agriculture and Forestry University,Xinyang 464000,Henan,China;College of Agriculture,Xinyang Agriculture and Forestry University,Xinyang 464000,Henan,China)
出处
《浙江农业学报》
北大核心
2025年第7期1407-1416,共10页
Acta Agriculturae Zhejiangensis
基金
河南省科技攻关项目(24210211165)
河南省高等学校重点科研计划项目(24B210011)
信阳农林学院青年教师科研基金项目(QN2021048,QN2021045)
信阳农林学院园艺植物资源与利用科技创新团队项目(XNKJTD-012)。
关键词
浙江红花油茶
ABCG5
转运蛋白
基因克隆
亚细胞定位
遗传转化
Camellia chekiangoleosa Hu
ABCG5
transporter
gene cloning
subcellular localization
genetic transformation
