摘要
Activated anti-oxidation reactions in cells partially diminish the anticancer effect of photodynamic therapy(PDT),significantly hindering efforts to increase the efficacy of PDT.The expression of transcription factor E2 related factor 2(Nrf2),an important redox-regulated transcription factor,can be downregulated by Nrf2 siRNA,leading to greatly enhanced PDT effects.However,the efficient co-delivery of photosensitizers and siRNAs remains a key problem because these agents are complex to synthesize,exhibit poor biocompatibility and load drugs with a low efficiency.Herein,we designed a carrier-free and extremely simple strategy to co-deliver a photosensitizer and Nrf2 siRNA to cancer cells.In this nanoplatform,an indocyanine green photosensitizer,siRNA and FeⅡwere self-assembled to form a spherical hybrid structure with a uniform size,high loading ratio and adjustable component ratio.The platform can effectively transfer photosensitizers and siRNAs into cells and effectively inhibit tumour growth in vivo.Overall,the self-assembly approach shows great potential for clinical application and provides a simple method to achieve photodynamic therapy and enhanced photothermal therapy.
基金
funded by the National Natural Science Foundation of China(51972003,52271127)
the National Key Technologies R&D Program(2022YFC2403203-3)
Intergovernmental International Cooperation Project of Beijing Municipal Science and Technology Commission(Z221100002722004).
