摘要
为探究旱作和水田土壤氧化亚氮还原菌丰度特征及其影响因素,使用实时荧光定量PCR方法,测定了江西省16个样点旱作花生和淹水稻田土壤氧化亚氮还原基因nosZⅠ和nosZⅡ丰度,并分析其主要影响因子。结果发现:旱作和水田土壤的nosZⅡ基因拷贝数均显著高于nosZⅠ,表明酸性农田土壤中nosZⅡ型氧化亚氮还原菌占据主导地位。再者,旱作土壤nosZⅠ的拷贝数为2.28×10^(8)copies·g^(-1),显著高于水田的1.33×10^(8)copies·g^(-1),表明nosZⅠ型氧化亚氮还原菌可能更喜好旱作土壤而非水田土壤,但旱作和水田土壤的nosZⅡ基因丰度无显著差异。旱作土壤中影响nosZⅠ和nosZⅡ基因拷贝数最主要的环境因子是总碳含量,这可能是由于氧化亚氮还原菌是异养微生物,总碳可为其提供碳源和能源;而有效磷含量和pH值则分别对水稻土nosZⅠ和nosZⅡ基因拷贝数的解释度最高,这可能是因为水稻土总碳含量较高,不是限制因子,而磷素和pH的影响更重要。综上,江西省旱作花生和水稻土壤氧化亚氮还原菌丰度的分布特征存在差异,nosZⅠ型氧化亚氮还原菌偏好旱作土壤,而nosZⅡ对旱地和水田无显著偏好性。
To explore the characteristics of nitrous oxide⁃reducing bacteria abundance in dryland and paddy soils and their influencing factors,real⁃time quantitative PCR was used to measure the abundance of nosZⅠand nosZⅡgenes in the soils of dryland peanut and flooded paddy fields from 16 sites in Jiangxi Province.The main influencing factors were analyzed.The results showed that the copy number of the nosZⅡgene in both dryland and paddy soils was significantly higher than that of nosZⅠ,indicating that nosZⅡ⁃type nitrous oxide⁃reducing bacteria dominated in acidic agricultural soils.The abundance of nosZⅠin dryland soils was 2.28×10^(8)copies·g^(-1),significantly high⁃er than that found in paddy soils(1.33×10^(8)copies·g^(-1)),indicating that nosZⅠ⁃type nitrous oxide⁃reducing bac⁃teria may prefer dryland soils over paddy soils.However,there was no significant difference in the abundance of nosZⅡgenes between dryland and paddy soils.In dryland soils,the total carbon content was the most important factor influencing the copy numbers of nosZⅠand nosZⅡgenes,possibly because nitrous oxide⁃reducing bacteria are heterotrophic microorganisms with total carbon as the carbon sources and energy.Meanwhile,available phos⁃phorus content and pH value were the most important factors explaining the variations of copy numbers of nosZⅠand nosZⅡgenes in paddy soils,likely because the total carbon content in paddy soils is high and not a limiting factor,making phosphorus and pH more influential.In summary,the abundance of nitrous oxide⁃reducing bacteria differed in dryland peanut and paddy soils in Jiangxi Province,with nosZⅠ⁃type nitrous oxide⁃reducing bacteria fa⁃voring dryland soils and nosZⅡshowing no significant preference.
作者
成于恒
冯蒙蒙
宛颂
邓米林
贺纪正
林永新
CHENG Yuheng;FENG Mengmeng;WAN Song;DENG Milin;HE Jizheng;LIN Yongxin(Cultivation Base of State Key Laboratory for Subtropical Mountain Ecology,Fuzhou 350007,China;School of Geographical Sciences,Fujian Normal University,Fuzhou 350007,China)
出处
《生态学杂志》
北大核心
2025年第8期2541-2547,共7页
Chinese Journal of Ecology
基金
国家自然科学基金项目(42077041,41930756)资助。
