摘要
目的 探讨炎症性肠病(IBD)患者肠道菌群分布特征及其与血浆外泌体微小RNA-214-3p(miR-214-3p)和miR-184的关系。方法 检测98例IBD患者(观察组)和同期100例健康体检者(对照组)的肠道菌群,采用实时荧光定量PCR检测血浆外泌体miR-214-3p和miR-184表达。比较两组肠道菌群数量、肠道菌群阳性率、血浆外泌体miR-214-3p和miR-184表达,采用Pearson相关分析观察组血浆外泌体miR-214-3p和miR-184表达与菌群数量的关系。采用ROC曲线评估血浆外泌体miR-214-3p和miR-184对IBD的诊断价值。结果 观察组双歧杆菌、拟杆菌、肠球菌、酵母菌和肠杆菌数量均高于对照组,真杆菌数量低于对照组(P<0.05)。观察组双歧杆菌和酵母菌菌群阳性率高于对照组,真杆菌菌群阳性率低于对照组(P<0.05)。观察组血浆外泌体miR-214-3p表达及血清IL-6、TNF-α、hsCRP水平高于对照组,血浆外泌体miR-184表达低于对照组(P<0.05)。血浆外泌体miR-214-3p表达与双歧杆菌、拟杆菌、肠球菌、酵母菌、肠杆菌数量及血清IL-6、TNF-α、hsCRP水平呈正相关,与真杆菌数量呈负相关(P<0.05);血浆外泌体miR-184表达与真杆菌数量呈正相关,与双歧杆菌、拟杆菌、肠球菌、酵母菌、肠杆菌数量及血清IL-6、TNF-α、hsCRP水平呈负相关(P<0.05)。血浆外泌体miR-214-3p、miR-184单独及联合诊断IBD的AUC分别为0.831、0.819和0.912(P<0.01)。结论 IBD患者血浆外泌体miR-214-3p表达升高和miR-184表达降低均与肠道菌群分布特征及炎症因子有关,可作为辅助诊断IBD的生物标志物。
Objective To explore the distribution characteristics of intestinal microflora in the patients with inflammatory bowel disease(IBD)and their relationship with miR-214-3p and miR-184 in plasma exosomes.Methods The intestinal microflora of 98 IBD cases(group A)and 100 health examinees(group C)in the same period were detected,and the expresssions of miR-214-3p and miR-184 in plasma exosomes were detected by real-time fluorescent quantitative PCR.The number of intestinal microflora,positive detection rate of intestinal microflora and the expresssions of miR-214-3p and miR-184 in plasma exosomes were compared between the two groups.Pearson correlation method was used to analyze the relationship of the expresssions of miR-214-3p and miR-184 in plasma exosomes with the number of intestinal microflora in group A.The diagnostic value of miR-214-3p and miR-184 in plasma exosomes for IBD was evaluated by ROC curve.Results The numbers of Bifidobacterium,Bacteroides,Enterococcus,Yeast and Enterobacteriaceae were higher,while the number of Eubacterium was lower in group A than those in group C(P<0.05).The positive rates of Bifidobacterium and Yeast flora were higher,while the positive rate of Eubacterium flora was lower in group A than those in group C(P<0.05).The expresssion of miR-214-3p in plasma and serum levels of IL-6,TNF-α,hsCRP in group A were higher,while the expresssions of miR-184 in plasma was lower in group A than those in group C(P<0.05).The expresssion of miR-214-3p in plasma exosomes was positively correlated with the numbers of Bifidobacterium,Bacteroides,Enterococcus,Yeast,Enterobacteriaceae and serum levels of IL-6,TNF-α,hsCRP,and negatively correlated with the number of Eubacterium(P<0.05).The expresssion of miR-184 in plasma exosomes was positively correlated with the number of Eubacterium,and negatively correlated with the numbers of Bifidobacterium,Bacteroides,Enterococcus,Yeast,Enterobacteriaceae and serum levels of IL-6,TNF-α,hsCRP(P<0.05).The AUC of miR-214-3p and miR-184 alone and in combination in plasma exosomes for diagnosing IBD were 0.831,0.819 and 0.912,respectively(P<0.01).Conclusion The increased expresssion of miR-214-3p and decreased expresssion of miR-184 in plasma exosomes of IBD patients are related to distribution characteristics of intestinal microflora and inflammatory factors,which can be used as the biomarkers in diagnosing IBD.
作者
袁玥旻
李珍
张秀峰
董丽丽
裘建明
李华铭
YUAN Yuemin;LI Zhen;ZHANG Xiufeng;DONG Lili;QIU Jianming;LI Huaming(Department of Anorectal Surgery,Hangzhou Third People's Hospital,Hangzhou 310000,CHINA)
出处
《江苏医药》
2025年第7期665-669,共5页
Jiangsu Medical Journal
基金
浙江省医药卫生科技计划项目(2019KY505)。
