摘要
采用整合基因组学与转录组学的研究策略,系统解析了粗山羊草、二穗短柄草、野生二粒小麦、硬粒小麦及乌拉尔图小麦等5种禾本科植物中DUF760基因家族的进化特征与生物学功能。结果表明,通过全基因组水平鉴定,共得到36个DUF760家族成员,其基因拷贝数在多倍体物种中呈显著扩增趋势。系统发育分析将其划分为类群Ⅰ和类群Ⅱ两个亚族,其中类群Ⅰ基因的外显子数目显著少于类群Ⅱ。顺式作用元件分析共检测到42类、2 180个调控元件。共线性分析显示,多倍体物种的基因保留率高于二倍体祖先物种。时空表达数据分析显示,TtDUF760基因在硬粒小麦胚乳发育过程中呈现动态表达模式,而二穗短柄草的BdDUF760基因则在胚乳组织中特异性高表达。在非生物胁迫响应方面,BdDUF760-01、BdDUF760-04、BdDUF760-05基因在干旱胁迫下表达量显著上调,硬粒小麦耐旱基因型TL5通过负向调控TtDUF760-04的表达响应复合胁迫。研究表明,DUF760基因家族通过亚功能化分化、结构域重组及动态表达调控等分子机制,在禾本科植物发育调控与环境适应中发挥重要作用。
By adopting a research strategy integrating genomics and transcriptomics,the evolutionary characteristics and biological functions of the DUF760 gene family in five gramineous plants,namely Aegilops tauschii,Brachypodium distachyon,Triticum turgidum subsp.durum and Triticum urartu,were systematically analyzed.The results showed that through genome-wide identification,a total of 36 members of the DUF760 family were identified,and their gene copy numbers showed a significant amplification trend in polyploid species.Phylogenetic analysis divided it into two subfamilies,GroupⅠand GroupⅡ,among which the number of exons of GroupⅠgenes was significantly less than that of GroupⅡ.A total of 42 types and 2180 regulating elements were detected in the analysis of cis-acting elements.Collinearity analysis revealed that the gene retention rate of polyploid species was higher than that of diploid ancestral species.Spatio-temporal expression data analysis showed that the TtDUF760 gene presented a dynamic expression pattern during the endosperm development of hard-grain wheat,while the BdDUF760 gene of short-stemmed grass was specifically highly expressed in the endosperm tissue.In terms of abiotic stress response,the expression levels of BdDUF760-01,BdDUF760-04,and BdDUF760-05 genes were significantly upregulated under drought stress.The drought-tolerant genotype TL5 of Triticum turgidum subsp.durum responded to compound stress by negatively regulating the expression of TtDUF760-04.The research results showed that the DUF760 gene family played a significant role in the developmental regulation and environmental adaptation of Poaceae plants through molecular mechanisms such as subfunctionalized differentiation,domain recombination,and dynamic expression regulation.
作者
李政
周思远
任星旭
李永珍
吴涛
胡宁
蒋雯
刘奕晗
蒋志凯
LI Zheng;ZHOU Siyuan;REN Xingxu;LI Yongzhen;WU Tao;HU Ning;JIANG Wen;LIU Yihan;JIANG Zhikai(Xinxiang Institute of Agricultural Sciences,Xinxiang Henan 453000,China)
出处
《种子》
北大核心
2025年第7期26-35,共10页
Seed
基金
国家现代农业小麦产业技术体系新乡小麦综合试验站(CARS-03-75)
河南省农业(小麦)良种联合攻关项目(2022010103)。
