摘要
将DNA纳米技术融入本科实验教学,设计了一个创新实验。该实验将DNA纳米开关的“打开”和“闭合”状态分别表示为0和1,通过加入RNA单链制备4种构型不同的DNA纳米开关,根据它们在琼脂糖凝胶电泳中的迁移速率不同实现对数字0-9的二进制表达,运用RNase A酶对RNA的特异性作用对密码进行加密,最后使用DNA单链解密并读取密码。通过该实验,成功地将信息学中的二进制算法融合到DNA纳米技术中,不仅使学生掌握了DNA纳米领域的相关知识和实验技能,而且让学生体验了一种新的信息加密和解密方法,取得了良好的教学效果。
We integrated DNA nanotechnology into experimental teaching and designed an innovative experiment.In our experiment,we utilized the"open"and"closed"states of a DNA nanoswitch to represent O and 1 respectively.By adding RNA single strands,we prepared four types of DNA nanoswitches.The binary expression of numbers O-9 is achieved through the differential migration rates of the switches in agarose gel electrophoresis,while the password is encrypted using RNase A's specific action on RNA.Subsequently,the password is decrypted and read using DNA single strands.This experiment successfully integrates binary algorithms from information science into DNA nanotechnology,enabling students not only to master relevant knowledge and experimental skills in DNA nanotechnology but also experience a new method of information encryption and decryption.
作者
段金伟
赵家婷
王璐瑶
宋哲
胡馨安
徐立言
崔林
DUAN Jin-Wei;ZHAO Jia-Ting;WANG Lu-Yao;SONG Zhe;HU Xin-An;XU Li-Yan;CUI Lin(College of Sciences,Chang'an University,Xi'an 710064,China;Shaanxi Weishinuo Biotechnology Co.Ltd,Xi'an 710304,China;School of Civil Engineering,Chang'an University,Xi'an 710061,China)
出处
《化学教育(中英文)》
北大核心
2025年第16期39-46,共8页
Chinese Journal of Chemical Education
基金
长安大学2023年度高等教育教学改革研究项目(BY202336)
2023年教育部产学合作协同育人项目(230719135707303)。
