摘要
目的:探讨下颌升支截骨术后乳酸脱氢酶A(lactate dehydrogenase A,LDHA)对牙槽骨改建的影响。方法:构建大鼠单侧下颌升支截骨模型,将其分为手术组和假手术组;选用小鼠RAW264.7细胞并诱导分化为破骨细胞,分为常氧组、低氧组、低氧+基因敲除(knockout,KO)组、低氧+siNC组、低氧+siLDHA组。通过抗酒石酸酸性磷酸酶(tartrate resistant acid phosphatase,TRAP)染色及免疫荧光染色检测局部牙槽骨内破骨细胞数目及LDHA表达水平;通过实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)、蛋白质印迹法和TRAP染色法,检测低氧条件下低氧诱导因子1α(hypoxia-inducible factor 1α,HIF-1α)对破骨细胞内LDHA表达的影响;通过免疫荧光染色检测LDHA表达水平对破骨特异性基因表达的影响。结果:下颌升支截骨术后牙槽骨内破骨细胞活跃,术后牙槽骨破骨细胞内LDHA表达增强。体外低氧环境下,破骨细胞内LDHA表达较常氧组增强,破骨分化能力亦随之增强;相较于低氧组,低氧+siLDHA组破骨相关特异性基因表达受到抑制。结论:下颌升支截骨术后LDHA可以通过促进破骨细胞形成加速牙槽骨改建。
Objective:To investigate the effect of lactate dehydrogenase A(LDHA)on alveolar bone remodeling after mandibular ramus osteotomy.Methods:The rat model of unilateral mandibular ramus osteotomy was established in vivo,and the rats were divided into the surgical group and the sham surgical group.Mouse RAW264.7 cells were induced to differentiate into osteoclasts and divided into normoxia group,hypoxia group,hypoxia+knockout(KO)group,hypoxia+siNC group,hypoxia+siLDHA group.The number of osteoclasts and LDHA expression levels in the local alveolar bone were detected using tartrate resistant acid phosphatase(TRAP)staining and immunofluorescence staining.The effects of hypoxia-inducible factor 1α(HIF-1α)on LDHA expression in osteoclasts under hypoxic conditions were assessed using real-time quantitative polymerase chain reaction(RT-qPCR),Western blotting,and TRAP staining.The impact of LDHA expression levels on the expression of osteoclast-specific genes was evaluated using immunofluorescence staining.Results:The osteoclasts in alveolar bone were active after mandibular ramus osteotomy,and the level of LDHA in alveolar osteoclasts increased after operation.Under hypoxic conditions in vitro,LDHA expression in osteoclasts was significantly increased compared to the normoxia group,and osteoclast differentiation capacity was also enhanced.Compared to the hypoxia group,the expression of osteoclast-specific genes was suppressed in the hypoxia+siLDHA group.Conclusion:LDHA after mandibular ramus osteotomy can accelerate alveolar bone remodeling by promoting osteoclast formation.
作者
郑宇杉
唐燚
康非吾
ZHENG Yushan;TANG Yi;KANG Feiwu(Shanghai Engineering Research Center of Tooth Restoration and Regeneration&Tongji Research Institute of Stomatology&Department of Oral and Maxillofacial Surgery,Shanghai Tongji Stomatological Hospital and Dental School,Tongji University,Shanghai 200072,China)
出处
《口腔颌面外科杂志》
2025年第4期260-267,共8页
Journal of Oral and Maxillofacial Surgery
基金
国家自然科学基金(82271013、82100955)
中国博士后科学基金项目(2021M692429)。
关键词
下颌升支截骨术
低氧
乳酸脱氢酶A
破骨细胞
牙槽骨改建
mandibular ramus osteotomy
hypoxia
lactate dehydrogenase A
osteoclast
alveolar bone remodeling
