摘要
目的建立溶剂洗脱-液相色谱法测定工作场所空气中三氯苯胺浓度。方法于2023年12月至2024年1月, 用玻璃纤维滤膜采集空气中2, 4, 5-三氯苯胺、2, 4, 6-三氯苯胺、3, 4, 5-三氯苯胺;经乙腈洗脱, 0.22 μm混合纤维素滤膜过滤后, 采用C8柱(250 mm×4.6 mm×5 μm)以乙腈和水(体积比为55∶45)等度洗脱, 二极管阵列(PDA)检测器进行检测, 对方法的检出限、精密度等指标进行分析。结果溶剂洗脱-液相色谱方法9 min内完成三氯苯胺异构体的分离测定, 线性范围为0.1~30 μg/ml, 方法检出限为0.005 μg/ml, 加标回收率为88%~98%, 相对标准偏差为0.3%~3.2%。结论三氯苯胺测定的溶剂洗脱-液相色谱方法简单高效, 回收率与精密度均符合工作场所有毒物质测定标准要求, 适用于工作场所中三氯苯胺的检测。
Objective To establish a solvent elution-liquid chromatography method for the determination of trichloroaniline concentration in the air of the workplace. From December 2023 to January 2024,2,4,5-trichloroaniline,2,4,6-trichloroaniline,and 3,4,5-trichloroaniline in the air were collected using glass fiber filter membranes.After elution with acetonitrile and filtration with a 0.22μm mixed cellulose filter membrane,isometric elution with acetonitrile and water(volume ratio 55∶45)was performed using a C8 column(250 mm×4.6 mm×5μm),and detection was carried out using a photo-diode array(PDA)detector.The detection limit,precision and other indicators of the method were analyzed.ResultsThe separation and determination of trichloroaniline isomers were completed within 9 minutes by the solvent elution-liquid chromatography method.The linear range was 0.1-30μg/ml,the detection limit of the method was 0.005μg/ml,the recoveries were 88%-98%,and the relative standard deviation was 0.3%-3.2%. The solvent elution-liquid chromatography method for the determination of trichloroaniline is simple and efficient.Both the recovery and precision meet the standard requirements for the determination of toxic substances in the workplace,and it is suitable for the detection of trichloroaniline in the workplace.
作者
陈炳奔
郑三燕
杨舒然
方晓丽
李瑞芬
Chen Bingben;Zheng Sanyan;Yang Shuran;Fang Xiaoli;Li Ruifen(Laboratory for Physicochemical Analysis,Wenzhou Center for Disease Control and Prevention(Wenzhou Health Supervision Office),Wenzhou 325000,China)
出处
《中华劳动卫生职业病杂志》
北大核心
2025年第7期539-542,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
温州市基础性科研项目(Y20240966)。
关键词
色谱法
液相
三氯苯胺
空气
工作场所
Chromatography,liquid
Trichloroaniline
Air
Workplace
