摘要
目的探讨乳腺乳头状肿瘤微生物组成特征,分析核梭杆菌属的丰度与良恶性肿瘤及其临床指标的相关性,明确影响恶性肿瘤发生的独立危险因素。方法回顾性纳入2020年1月至2023年12月武汉大学人民医院乳腺甲状腺外科72例经术后病理确诊的乳腺乳头状肿瘤患者,其中良性25例,恶性47例。采集甲醛溶液固定石蜡包埋组织样本,采用16S rRNA基因测序分析肿瘤组织菌群组成与多样性,比较良恶性肿瘤的α多样性指数(Chao1、Faith_pd、Observed_species、Shannon),并采用Kruskal-Wallis检验筛选差异菌属。通过单因素、多因素Logistic回归分析评估核梭杆菌属与恶性肿瘤风险的关联。结果恶性肿瘤组α多样性显著高于良性组[Chao1:215.282(100.823,342.457)比616.534(356.234,752.416),Z=-2.374,P=0.018;Faith_Pd:48.888(23.390,60.698)比105.198(58.700,136.172),Z=-2.387,P=0.017;Observed_species:191.900(95.600,339.800)比612.800(343.700,749.700),Z=-2.734,P=0.018;Shannon:2.729(2.426,3.967)比7.016(3.781,8.932),Z=-3.089,P=0.002]。良性组优势菌为乳杆菌属(0.962%)、不动杆菌属(2.250%),恶性组富集普雷沃氏菌属(0.668%)、葡萄球菌属(0.160%)、红球菌属(0.016%)。两组核梭杆菌属丰度差异有统计学意义[0.000079(0.000026,0.000245)比0.000512(0.000168,0.001560),H=4.464,P=0.035]。Logistic回归分析显示,手术年龄>50岁(OR=12.520,95%CI:3.150~49.739,P=0.001)、肿块体积>1 cm^(3)(OR=6.598,95%CI:1.681~25.901,P=0.007)、存在核梭杆菌属(OR=5.943,95%CI:1.154~30.600,P=0.033)为恶性肿瘤的独立危险因素。结论乳腺乳头状肿瘤的菌群特征与良恶性密切相关,核梭杆菌属可能为其恶性风险的潜在生物标志物,这为靶向菌群干预提供理论依据。
Objective To investigate the microbial composition of breast papillary tumors,analyze the correlation between Fusobacterium abundance and tumor malignancy as well as clinical parameters,and identify independent risk factors for malignancy.Methods A total of 72 patients with pathologically confirmed breast papillary tumors who underwent surgery in the Department of Breast and Thyroid Surgery,Renmin Hospital of Wuhan University between January 2020 and December 2023 were retrospectively enrolled,including 25 benign and 47 malignant cases.Formalin-fixed paraffin-embedded tissue samples were collected for 16S rRNA gene sequencing to assess microbial diversity and composition.Alpha diversity indexes(Chao1、Faith_pd,Observed_species,Shannon)were compared between benign and malignant groups,and Kruskal-Wallis test was used to identify significantly different taxa.The association between Fusobacterium and malignancy risk was evaluated using univariate and multivariate logistic regression.Results The malignant group showed significantly higher alpha diversity compared with the benign group[Chao1:215.282(100.823,342.457)vs 616.534(356.234,752.416),Z=-2.374,P=0.018;Faith_Pd:48.888(23.390,60.698)vs 105.198(58.700,136.172),Z=-2.387,P=0.017;Observed_species:191.900(95.600,339.800)vs 612.800(343.700,749.700),Z=-2.374,P=0.018;Shannon:2.729(2.426,3.967)vs 7.016(3.781,8.932),Z=-3.089,P=0.002]Dominant genera in the benign group included Lactobacillus(0.962%)and Acinetobacter(2.250%),while Prevotella(0.668%)Staphylococcus(0.160%),and Rhodococcus(0.016%)were enriched in the malignant group.The abundance of Fusobacterium was significantly higher in the malignant group than in the benign group[0.000079(0.000026,0.000245)vs 0.000512(0.000168,0.001560),H=4.464,P=0.035].Logistic regression analysis revealed that age>50 years(OR=12.520,95%CI:3.150~49.739,P=0.001),tumor volume>1 cm^(3)(OR=6.598,95%CI:1.681~25.901,P=0.007),and presence of Fusobacterium(OR=5.943,95%CI:1.154~30.600,P=0.033)were independent risk factors for malignancy.Conclusion The microbial composition of papillary breast tumors is closely associated with tumor malignancy.Fusobacterium may serve as a potential microbial biomarker for malignancy,providing a theoretical basis for microbiota-targeted intervention.
作者
钟凯欣
胡嘉薇
罗梓轩
熊尧
陈创
Zhong Kaixin;Hu Jiawei;Luo Zixuan;Xiong Yao;Chen Chuang(Department of Breast and Thyroid Surgery,Renmin Hospital of Wuhan University,Wuhan 430000,China)
出处
《中华乳腺病杂志(电子版)》
2025年第3期154-159,共6页
Chinese Journal of Breast Disease(Electronic Edition)
基金
湖北省自然科学基金计划(一般面上项目)(2023AFB701)。
关键词
微生物群
乳腺肿瘤
癌
乳头状
核梭杆菌属
Microbiota
Breast neoplasms
Carcinoma,papillary
Fusobacterium
