摘要
目的 建立HPLC法用于测定翁沥通胶囊药材组成中木犀草苷、芦荟大黄素、栀子苷和甘草苷4种成分的含量。方法 采用Agilent ZORBAX Eclipse Plus C18色谱柱分离,柱温为室温,流速设定为1.0 mL·min-1,检测波长:木犀草苷351 nm、芦荟大黄素254 nm、栀子苷和甘草苷237 nm,进样量为10μL,采用HPLC行定性、定量检测。结果 木犀草苷、芦荟大黄素、栀子苷和甘草苷分别在质量浓度为0.03924~3.92400μg·mL^(-1)、0.03900~3.90000μg·mL^(-1)、0.02943~2.94300μg·mL^(-1)和0.01904~1.90400μg·mL^(-1)内具有良好的线性;加标平均回收率分别为99.63%、100.41%、100.04%和99.84%;方法 重复性RSD分别为0.98%、1.30%、1.00%和1.10%;供试品溶液在24 h内稳定。结论 实验建立的HPLC法具有前处理简单、分析时间短、检测结果 准确等优点,适用于翁沥通胶囊的质量控制。
AIM A rapid high-performance liquid chromatography(HPLC)method was developed to detect the content of 4 components,namely luteoloside,aloe-emodin,geniposide and liquiritin,in the medicinal composition of Wenglitong capsules.METHODS Separation was performed using an Agilent ZORBAX Eclipse Plus C18 column maintained at room temperature,with a mobile phase flow rate of 1.0 mL-min''.Detection wavelengths were set at 351 nm for luteoloside,254 nm for aloe-emodin,and 237 nm for simultaneous monitoring of geniposide and liquiritin.An injection volume of 10OμL was employed,and qualitative and quantitative analyses were performed using HPLC detection.RESULTS Luteoloside,aloe-emodin,gardenin and liquiritin exhibited good linearity within the ranges of 0.039240-3.92400μg'mL-,0.03900-3.90000μg·mL^(-1),0.02943-2.94300μg·mL^(-1)and 0.01904-1.90400μg·mL^(-1),respectively.The average recovery rates were 99.63%,100.41%,100.04%and 99.84%,respectively.The reproducibility RSDs were 0.98%,1.30%,1.00%and 1.10%,respectively.The test solution remained stable for 24 h.CONCLUSION TThe findings indicate that the method offers advantages such as simple pretreatment,reduced analysis time,and precise detection results,which is suitable for the quality control of Wenglitong capsules.
作者
梁银洪
陈浩
周杰鑫
余红花
谭本仁
LIANG Yinhong;CHEN Hao;ZHOU Jiexin;YU Honghua;TAN Benren(Department of Pharmacy,Nuclear Industry 419 Hospital,Shaoguan 512028,China)
出处
《中国临床药学杂志》
2025年第7期493-497,共5页
Chinese Journal of Clinical Pharmacy
基金
广东省医学科学技术研究基金项目(编号201811611510970)。
