摘要
目的:研究不同阶段牙髓炎中牙髓血清的蛋白质表达差异。方法:收集9例牙髓炎患者的牙髓血清,分为早期(initial pulpitis,IP)、轻中度(mild to moderate pulpitis,MP)和重度牙髓炎(severe pulpitis,SP)三组。通过蛋白质组学技术筛选差异蛋白。使用Metascape数据库进行功能注释和富集分析,并用STRING数据库分析差异蛋白的相互作用。结果:与IP组相比,MP组有20个蛋白上调,23个蛋白下调;SP组有1个蛋白上调,44个蛋白下调。与MP组相比,SP组有9个蛋白上调和15个蛋白下调。差异蛋白主要涉及细胞对压力的响应、适应性免疫应答和Parkin-泛素蛋白酶体系统等关键生物过程。蛋白质相互作用网络分析发现CCT6A等多个关键功能节点。结论:本研究通过蛋白质组学分析了早期、轻中度与重度牙髓炎之间牙髓血清蛋白质的表达差异,可为牙髓炎的精准诊断和临床决策提供理论依据。
PURPOSE:To investigate the differentially expressed proteins in dental pulp serum of pulpitis at different stages.METHODS:Dental pulp serum was collected from 9 pulpitis patients and categorized into three groups:initial pulpitis(IP),mild to moderate pulpitis(MP)and severe pulpitis(SP).Proteomics technology was utilized to screen for differentially expressed proteins.Functional annotation and enrichment analysis were then conducted using the Metascape database,while protein-protein interaction analysis was performed with the STRING database.RESULTS:Compared with the IP group,the MP group had 20 upregulated proteins and 23 downregulated proteins,while the SP group had one upregulated protein and 44 downregulated proteins.Compared to the MP group,the SP group had 9 upregulated proteins and 15 downregulated proteins.The differential proteins were mainly involved in key biological processes such as cellular response to stress,adaptive immune response,and the Parkin-ubiquitin proteasomal system.Protein-protein interaction network analysis identified several key functional nodes,including CCT6A.CONCLUSIONS:This study analyzed the differential expression of proteins in dental pulp serum between early,mild to moderate,and severe pulpitis,providing a theoretical basis for accurate diagnosis and clinical decision-making of pulpitis.
作者
隋昕
徐华兴
李佳洋
秦志明
马智菲
周荣睿
周强强
韦晓玲
Sui Xin;Xu Huaxing;Li Jiayang;Qin Zhiming;Ma Zhifei;Zhou Rongrui;Zhou Qiangqiang;Wei Xiaoling(Department of Operative Dentistry and Endodontics,Shanghai Stomatological Hospital and School of Stomatology,Fudan University,Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases,Fudan University.Shanghai 200001,China)
出处
《上海口腔医学》
2025年第4期346-353,共8页
Shanghai Journal of Stomatology
基金
上海市卫生健康委员会临床研究专项(202140503)
上海市口腔医院人才专项(SHH-2022-YJ-07)
上海市口腔医院院级基金(SSH-2023-01,SSH-2023-02)。
