摘要
枸杞多糖(Lycium barbarum polysaccharides,LBPs)是宁夏枸杞发挥明目功效的主要活性物质,本研究基于活性追踪的策略,构建无金属芬顿反应降解LBPs的新方法,揭示不同组分LBPs的初级结构和明目活性。采用超声波辅助H_(2)O_(2)/抗坏血酸降解反应构建了无金属芬顿反应降解LBPs的新方法,对具有显著明目活性的LBPs进行降解。通过调控降解时间(0.5、1、3 h),制备了3种多糖降解组分LBPs-1、LBPs-2和LBPs-3。通过表征LBPs及其降解产物,结果表明LBPs-3分子量最低、阿拉伯半乳聚糖及糖醛酸含量最高。生物活性实验发现,LBPs-3具有最佳的明目活性,为阐明LBPs中明目药效物质基础提供理论依据。
Lycium barbarum polysaccharides(LBPs)are the main active substances in Lycium barbarum L.for its ability to improve eyesight.This article constructs a new method for metal-free Fenton reaction degradation of LBPs,revealing the primary structure and eyesight-improving activity of LPBs with different components.A new method for degrading LBPs was constructed using an ultrasound-assisted H_(2)O_(2)/ascorbic acid degradation reaction,which established a metal-free Fenton reaction.This method was applied to degrade LBPs,which exhibit significant eyesight-improving activity.By adjusting the degradation time(0.5,1,3 h),three different degraded components of LBPs were prepared.Characterization of LBPs and its degradation products revealed that LBPs-3 had the low molecular weight and the highest content of arabinogalactans and uronic acids.Biological activity experiments indicated that LBPs-3 possessed the most potent eyesight-improving activity.The optimal LBPs component with the best eyesight-improving activity was screened,providing a theoretical basis for clarifying the pharmacodynamic substance basis of eyesight-improving activity of LBPs.
作者
王小芳
曹妤
刘建飞
裴栋
曲清莉
WANG Xiao-fang;CAO Yu;LIU Jian-fei;PEI Dong;QU Qing-li(Gansu Pharmaceutical Group Technology Innovation Research Institute Co.,Ltd.;CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China)
出处
《天然产物研究与开发》
北大核心
2025年第8期1441-1448,共8页
Natural Product Research and Development
基金
甘肃省企业创新联合体绩效评价应用项目(24ZD17FA003)
中央引导地方科技发展资金项目(23ZYQF0307)
甘肃省科技重大专项(22ZD6FA021)
国有资本经营预算支持省属企业科研项目计划(2024GZ021)。
关键词
枸杞多糖
超声
无金属芬顿反应
明目活性
Lycium barbarum polysaccharides
ultrasound
metal-free Fenton reaction
eyesight-improving activity
