摘要
目的蜱为田鼠巴贝斯虫的传播媒介,其作为巴贝斯虫媒介的具体机制尚不明确。本研究旨在研究蜱自噬分子对田鼠巴贝斯虫增殖的影响。方法通过长角血蜱叮咬感染与未感染田鼠巴贝斯虫的小鼠实验模型,收集蜱材料进行蛋白质差异组学分析,筛选具有差异表达的长角血蜱自噬相关分子Haemaphysalis longicornis ATG8(HlATG8),完成其编码基因克隆、蛋白表达及多克隆抗体的制备。利用RNAi干扰技术敲低HlATG8基因。结果鉴定了长角血蜱自噬分子ATG8。蜱感染田鼠巴贝斯虫后,HlATG8蛋白显著上调。敲低HlATG8基因,可以显著增加巴贝斯虫感染量。结论长角血蜱自噬分子ATG8抑制巴贝斯虫的体内增殖。
Objective Ticks serve as vectors for transmitting Babesia microti.However,the specific mechanism remains unclear.This study aimed to investigate the effect of tick autophagy molecules on the proliferation of Babesia microti.Methods An experimental model of infected and uninfected mice was used to collect tick materials for proteomic analysis to identify differentially expressed autophagy-related molecules in Haemaphysalis longicornis.The cloning of the HlATG8 gene,protein expression,and production of polyclonal antibodies were completed.The HlATG8 gene was then knocked down using RNAi interference technology.Results The tick autophagy molecule,HlATG8,was identified and found to be significantly upregulated in ticks infected with Babesia microti.The load of Babesia microti in ticks increased significantly following the knockdown of the HlATG8 gene.Conclusions The tick autophagy molecule in Hae.longicornis,HlATG8,inhibits the proliferation of Babesia.
作者
龚凤君
曹杰
周勇志
王亚楠
张厚双
周金林
GONG Feng-jun;CAO Jie;ZHOU Yong-zhi;WANG Ya-nan;ZHAHG Hou-shuang;ZHOU Jin-lin(Key Laboratory of Animal Parasitology,Ministry of Agriculture,Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China)
出处
《寄生虫与医学昆虫学报》
2025年第2期93-98,共6页
Acta Parasitologica et Medica Entomologica Sinica
基金
国家重点研发计划项目(2022YFD1800200)。
关键词
田鼠巴贝斯虫
长角血蜱
自噬
ATG8
Babesia microti
Haemaphysalis longicornis
Autophagy
ATG8
