摘要
通过探究荒漠绿洲过渡带不同植被类型对土壤细菌群落结构及代谢功能的影响,为荒漠生态脆弱区植被重建的物种配置和管理提供科学理论依据.中国西北荒漠-绿洲过渡带以泡泡刺(Ns)和沙拐枣(Cm)为天然植被,梭梭(Ha)和花棒(Hs)为人工植被,Ha-Cm为混合群落.了解典型植被类型下土壤微生物群落结构和功能,对荒漠植被精准管理和可持续利用具有重要意义.采用高通量测序,探究不同植被类型对土壤细菌结构、多样性和代谢途径的影响,考察驱动细菌群落结构变化的关键因子.人工植被和天然植被生长显著提高土壤细菌群落丰富度和多样性,但无显著差异;宏基因组分析显示,各植被中均存在相同优势细菌门但比例不同,放线菌门、变形菌门和绿弯菌门为优势菌门,占细菌总数的65.12%~78.68%;主坐标分析(PCoA)显示,人工植被的土壤细菌结构差异显著,而天然植被的相似;不同植被群落土壤细菌代谢途径相似;土壤有机碳(SOC)、速效磷(AP)和pH是影响细菌功能结构的重要因子.研究结果有助于深入理解干旱荒漠生态脆弱区植物-土壤相互作用.
The aim of this study was to explore the effects of different vegetation types on soil bacterial community structure and metabolic function in an oasis-desert ecotone and provide scientific theoretical basis for species allocation and management of vegetation reconstruction in arid desert ecosystem.Nitraria sphaerocarpa Maxim and Calligonum mongolicum Turcz are the main natural vegetation,Haloxylon ammodendron Bunge,and Hedysarum scoparium Fisch are the primary artificial vegetation,and Ha-Cm are the main mixed community in an oasis-desert ecotone in northwest China.Understanding soil microbial community structure and function under typical vegetation types is crucial for accurate management and sustainable use of desert vegetation.We used high-throughput sequencing technology to explore the effects of different vegetation types on the structure,diversity,and metabolic pathways of soil bacteria and to investigate the key factors driving the change of soil bacterial community structure.The results showed that the growth of artificial vegetation and natural vegetation significantly increased the richness and diversity of the soil bacterial community,but no significant difference was observed.Metagenomic analysis showed that the same dominant bacterial phyla existed in all soils,but the proportions were different.Actinobacteriota,Proteobacteria,and Chloroflexi were the absolute dominant bacterial phyla,accounting for 65.12%-78.68%of the total bacteria.Principal co-ordinates analysis showed significant differences in soil bacterial community structure in the planted forest but similarities in the natural forest.The metabolic pathways of soil bacteria in different vegetation communities were similar.Soil organic carbon(SOC),available phosphorus(AP),and pH were important abiotic factors affecting the functional structure of bacteria.The findings are helpful for furthering the understanding of plant-soil interaction in ecologically fragile deserts.
作者
安芳娇
牛子儒
刘婷娜
苏永中
AN Fang-jiao;NIU Zi-ru;LIU Ting-na;SU Yong-zhong(School of Civil Engineering,Lanzhou University of Technology,Lanzhou 730050,China;Shaanxi Provincial Land Engineering Construction Group,Xi'an 710075,China;Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou 730000,China)
出处
《环境科学》
北大核心
2025年第8期5360-5368,共9页
Environmental Science
基金
甘肃省青年科技基金项目(25JRRA912)
秦创原引用高层次创新创业人才项目(QCYRCXM-2022-299)。
关键词
干旱区
荒漠-绿洲过渡带
植被类型
土壤细菌结构
代谢功能
arid region
oasis-desert ecotone
vegetation type
soil bacterial structure
metabolic function
