摘要
为加快旱地小麦优质种质资源的创制,丰富遗传多样性,本研究采用甲基磺酸乙酯(EMS)诱变技术构建了小麦长6990突变体库,并对其表型性状和高分子量麦谷蛋白亚基(HMW-GS)进行了系统筛选。通过对M_(2)群体中20331个单株进行田间表型性状统计分析,共发现3244个突变单株,涵盖茎秆、叶片、芒、穗型、育性和生育期等方面田间表型特征,总突变率为15.96%。此外,对收获的2064份突变材料进行籽粒表型分析,筛选出25份籽粒突变材料,占突变材料的1.21%,突变类型包括粒长、粒色和饱满度等。通过对2064份突变材料进行SDS-PAGE鉴定,共筛选出23份HMW-GS缺失突变材料,占突变材料的1.11%,表现为Glu-B1和Glu-D1位点中Bx7、By9、Dx5、Dy10单亚基缺失。本研究采用EMS诱变技术,创制了类型丰富的表型突变体和HMW-GS突变材料,有效消除了遗传背景差异对小麦品质性状的潜在干扰,为旱地小麦品质育种提供了宝贵的遗传资源,并为深入理解小麦品质性状与遗传变异之间的关系提供了重要的基础数据。
To accelerate the creation of high-quality germplasm resources for drought-resistant wheat and enrich genetic diversity,this study constructed a mutant library of wheat Chang 6990 using EMS(Ethyl methanesulfonate)mutation technology and systematically screened its phenotypic traits and high molecular weight glutenin subunits(HMW-GS).A field phenotypic analysis of 20331 individual plants from the M2 population identified 3244 mutant plants,covering fields phenotypic traits of stem,leaf,awn,spike type,fertility,and growth period,with a total mutation rate of 15.96%.Additionally,a grain phenotypic analysis of 2064 harvested mutant materials revealed 25 grain mutants,accounting for 1.21%of the mutant materials,with mutation types including grain length,grain color,and fullness.Through SDS-PAGE identification of 2064 mutant materials,a total of 23 HMW-GS deletion mutants were screened,which showed the deletion of Bx7,By9,Dx5 and Dy10 in Glu-B1 and Glu-D1 loci.In this study,EMS mutagenesis technology was used to create abundant phenotypic mutants and HMW-GS mutant materials,which effectively eliminated the potential interference of genetic background differences on wheat quality traits,provided valuable genetic resources for dryland wheat quality breeding,and provided important basic data for in-depth understanding of the relationship between wheat quality traits and genetic variation.
作者
王泽宇
王闪闪
温宏伟
王裕智
张晋辉
单皓
闫金龙
张俊灵
杨斌
WANG Zeyu;WANG Shanshan;WEN Hongwei;WANG Yuzhi;ZHANG Jinhui;SHAN Hao;YAN Jinlong;ZHANG Junling;YANG Bin(Institute of Wheat Research,Shanxi Agricultural University,Linfen 041000;College of Agriculture,Shanxi Agricultural University,Jinzhong 030801;Institute of Millet Research,Shanxi Agricultural University,Changzhi 046000)
出处
《植物遗传资源学报》
北大核心
2025年第9期1754-1763,共10页
Journal of Plant Genetic Resources
基金
山西省基础研究计划(202103021223156)
山西省农业关键核心技术攻关(NYGG26-1)
山西省科技成果转化引导专项(202304021301052)。
