摘要
考察高效液相色谱法和离子色谱法两种方法检测化妆品原料乙酰基六肽-8中醋酸和三氟乙酸结果的一致性。对高效液相色谱法和离子色谱法测定醋酸和三氟乙酸的方法进行改进和优化,对专属性、线性范围、检出限、定量下限、回收率、精密度、重复性和稳定性等方法学指标作比对,并对15批次乙酰基六肽-8原料中醋酸和三氟乙酸的测定结果进行统计学分析。结果表明,两种方法均有良好的专属性,离子色谱法的线性范围较高效液相色谱法更宽,且检出限和定量下限比高效液相色谱法更低,但是高效液相色谱法的精密度更好。两种方法均能较好地对乙酰基六肽-8中醋酸和三氟乙酸进行定性和定量分析,经配对t检验处理,两种方法对酰基六肽-8中醋酸的测定结果无统计学差异(|t|=0.200,P>0.05)。两种方法各有优势,可根据实际情况选择适合的测定方法。
The study aims to investigate the consistency of analysis results of acetic acid and trifluoroacetic acid in cosmetic raw material acetyl hexapeptide-8 by high performance liquid chromatography(HPLC)and ion chromatography(IC).The methods of determination of acetic acid and trifluoroacetic acid by HPLC and IC were improved and optimized.The specificity,linear range,detection limit,quantitation limit,recovery,precision,repeatability and stability were compared.The results of 15 batches acid and trifluoroacetic acid in acetyl hexapeptide-8 raw materials were analyzed statistically.The results show that both two methods have good specificity,and the linear range of IC method is wider than that of HPLC method,with lower detection and quantification limits.However,the precision of HPLC method is better.Both two methods can be used for qualitative and quantitative analysis of acetic acid and trifluoroacetic acid in acetyl hexapeptide-8.There is no statistical difference between the testing results of HPLC and IC to determine acetic acid in acetyl hexapeptide-8(|t|=0.200,P>0.05).Because two methods have their own advantages,the appropriate analysis method can be selected according to the actual situation.
作者
陈岑
戴小敏
颜琳琦
程巧鸳
Cen Chen;Xiaomin Dai;Linqi Yan;Qiaoyuan Cheng(NMPA Key Laboratory for Animal Alternative Testing Technology of Cosmetics,Zhejiang Institute for Food and Drug Control,Hangzhou,Zhejiang310052,China)
出处
《日用化学工业(中英文)》
北大核心
2025年第8期1078-1084,共7页
China Surfactant Detergent & Cosmetics
基金
2023年《化妆品安全技术规范》制修订项目
浙江省药品监督管理局科技计划项目(2022001)。
关键词
醋酸
三氟乙酸
高效液相色谱法
离子色谱法
乙酰基六肽-8
acetic acid
trifluoroacetic acid
high performance liquid chromatography
ion chromatography
acetyl hexapeptide-8
