摘要
目的探讨金黄色葡萄球菌临床分离株的溶血表型。方法采用三点接种法检测105株临床样本分离金黄色葡萄球菌的溶血表型;实时荧光定量聚合酶链式反应检测4种溶血素基因(hla、hlb、hlc和hld)mRNA表达量;VITEK 2 GP639药敏卡检测常用抗生素耐药性;DNA凝胶电泳法检测mecA、sea、tst和pvl基因携带率;微孔板成膜结晶紫染色法检测生物膜形成能力;CCK-8法检测巨噬细胞毒性。结果金黄色葡萄球菌临床分离株中检出7种溶血表型。不同溶血表型金黄色葡萄球菌临床分离株在溶血素基因mRNA表达量、常用抗生素耐药性、毒力基因携带率、生物膜形成能力、小鼠巨噬细胞毒性作用等方面均有差异(P<0.05)。结论金黄色葡萄球菌临床分离株的溶血表型多样,应当引起微生物检验、临床治疗和院感防控等多维度重点关注。
Objective To investigate the hemolytic phenotypes of Staphylococcus aureus clinical isolates.Methods The hemolytic phenotypes of 105 Staphylococcus aureus isolates were analyzed and summarized using the three-point inoculation method.Real-time fluorescence quantitative PCR was used to measure the mRNA expression levels of four hemolysin genes(hla,hlb,hlc,and hld);The VITEK 2 GP639 antimicrobial susceptibility card was used to detect resistance to commonly used antibiotics;DNA gel electrophoresis was performed to determine the prevalence of the mecA,sea,tst,and pvl genes;The microtiter plate crystal violet staining method was used to assess biofilm formation ability;The CCK-8 assay was used to evaluate cytotoxicity against macrophages.Results Seven hemolytic phenotypes were identified among the Staphylococcus aureus clinical isolates.Differences were found among Staphylococcus aureus clinical isolates with different hemolytic phenotypes in terms of mRNA expression levels of hemolysin genes,antibiotic resistance,virulence gene prevalence,biofilm formation ability,and cytotoxicity to mouse macrophages(P<0.05).Conclusion Staphylococcus aureus clinical isolates exhibit diverse hemolytic phenotypes,which should be a focus across multiple dimensions,including microbiological testing,clinical treatment,and nosocomial infection prevention and control.
作者
高菊
翁胜男
冷贵云
李昕
姚杰
周强
唐伟
Gao Ju;Weng Shengnan;Leng Guiyun;Li Xin;Yao Jie;Zhou Qiang;Tang Wei(Dept of Clinical Laboratory,The Second Affiliated Hospital of Anhui Medical University,Hefei 230601)
出处
《安徽医科大学学报》
北大核心
2025年第7期1312-1319,共8页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金项目(编号:82102460)。
关键词
金黄色葡萄球菌
临床分离株
溶血表型
溶血素
耐药性
毒力
Staphylococcus aureus
clinical isolate
hemolytic phenotype
hemolysin
antibiotic resistance
virulence
