摘要
为探究轮胎微塑料(TP)对土壤酶活性和微生物群落的影响,以农田土壤为研究对象,通过56 d室内培养试验和高通量测序揭示不同浓度(0.1%、1%和10%)TP对土壤细菌群落的影响。结果表明,添加TP提高了土壤pH,降低了铵态氮和硝态氮含量。TP对土壤酶活性的影响主要表现为过氧化氢酶活性提高,以及蔗糖酶和脲酶活性降低。添加10%TP显著提高了土壤细菌的多样性,而添加1%和0.1%TP均无显著影响。添加1%和10%TP显著影响土壤细菌在门水平上的群落组成,但添加0.1%TP无明显影响。建议加强对土壤环境中中高浓度TP污染的管控和修复,以降低其对土壤微生物群落的影响。
To explore the effects of tire microplastics(TP)on soil enzyme activity and microbial communities,a 56-day indoor cultivation experiment was conducted using farmland soil as the research object,and high-throughput sequencing was utilized to reveal the impact mechanism of different concentrations(0.1%,1%and 10%)of TP on soil bacterial communities.The results indicate that the addition of TP increases soil pH and reduces the contents of ammonium nitrogen and nitrate nitrogen in the soil.TP affects soil enzyme activity,mainly by enhancing peroxidase activity and reducing sucrase and urease activity.The addition of high-concentration(10%)TP significantly increases the diversity of soil bacteria,while both medium and low concentrations(0.1%and 1%)have no significant effect.The addition of TP at concentrations of 1%and 10%significantly affect the community composition of soil bacteria at the phylum level,while the addition of TP at concentration of 0.1%has no obvious effect.It is suggested to strengthen the control and remediation of medium and high concentration TP pollution in the soil environment to reduce its impact on soil microbial communities.
作者
周长瑞
王晨曦
王云秋
胡慧
顾效纲
李国丰
刘彪
李松亚
ZHOU Chang-rui;WANG Chen-xi;WANG Yun-qiu;HU Hui;GU Xiao-gang;LI Guo-feng;LIU Biao;LI Song-Ya(School of Municipal and Environmental Engineering,Henan University of Urban Construction,Pingdingshan 467036,China;Henan Key Laboratory of Water Pollution Control and Rehabilitation Technology,Pingdingshan 467036,China)
出处
《河南城建学院学报》
2025年第4期101-107,共7页
Journal of Henan University of Urban Construction
基金
河南省科技攻关项目(242102320076,232102321044)
河南省高等学校重点研发项目(24B610001)。
关键词
轮胎微塑料
土壤酶活性
细菌群落
高通量测序
tire microplastics
soil enzyme activity
bacterial community
high-throughput sequencing
