摘要
苦瓜是我国传统的瓜类蔬菜,品种资源遗传背景狭窄,亟需通过野生种质资源拓宽遗传多样性。本研究以小果型野生亚种自交系NJ与普通亚种自交系Tan构建的F2群体为材料,基于重测序bin标记遗传图谱,利用MQM(Multiple-QTL model)方法对果长、果径、果形指数、果形、果重5个性状进行QTL定位。相关性分析表明,除果径与果形、果形指数与果重外,各性状间均存在显著相关性。重测序基因分型产生1921个bin标记,其中1563个被整合到亚种间遗传图谱,覆盖11条染色体,总遗传距离为1556.91 cM,标记间平均距离为1.00 cM。遗传图谱与物理图谱间比较显示苦瓜两个亚种在MC08号染色体存在一个约11 Mb的倒位区域。共有7个QTL达到了LOD检测阈值,其中果长2个(qFL5.1、qFL7.1)、果形指数2个(qFSI1.1、qFSI4.1)、果重2个(qFW5.1、qFW6.1)、果形1个(qFS5.1);果长qFL5.1、果形qFS5.1、果重qFW5.1形成一个一致性的主效QTL区间。研究结果有助于揭示苦瓜两个亚种基因组的变异特征以及为亚种间种质创新和果实相关性状基因挖掘奠定基础。
Bitter gourd(Momordica charantia L.)is a traditional melon vegetable in China,but its cultivated varieties suffer from a narrow genetic background,necessitating the expansion of genetic diversity through distance hybridization with wild germplasm.In this study,an F2 population was generated from a cross between the small-fruited wild subspecies inbred line NJ and the common subspecies inbred line Tan.QTL mapping was performed for five fruit-related traits(fruit length,fruit diameter,fruit shape index,fruit shape,and fruit weight)using a bin marker genetic map through genotyping-by-sequencing,with the MQM(Multiple-QTL model)method.Significant correlations were observed among most traits,except between fruit diameter and fruit shape,as well as between fruit shape index and fruit weight.1563 of 1921 bin markers were integrated into an inter-subspecies genetic map,spanning 1556.91 cM across 11 chromosomes,with an average genetic interval of 1.00 cM.Comparative analysis between the genetic and physical maps revealed approximately 11 Mb inversion on chromosome MC08 between the two subspecies.A total of seven QTL exceeding the LOD threshold were identified,including two for fruit length(qFL5.1,qFL7.1),two for fruit shape index(qFSI1.1,qFSI4.1),two for fruit weight(qFW5.1,qFW6.1),and one for fruit shape(qFS5.1).Notably,qFL5.1,qFS5.1 and qFW5.1 were mapped to a consensus major-effect QTL interval.The findings of this study contribute to our understanding of the genomic variation between the two subspecies of bitter gourd and provide the foundation for inter-subspecies germplasm innovation and gene mining of fruit-related traits.
作者
李炯
徐彬其
梁梓兴
曹振强
侯冰冰
洪宇
程蛟文
胡开林
崔竣杰
LI Jiong;XU Binqi;LIANG Zixing;CAO Zhenqiang;HOU Bingbing;HONG Yu;CHENG Jiaowen;HU Kailin;CUI Junjie(School of Agricultural and Biological Engineering,Foshan University,Foshan 528225,Guangdong;College of Horticulture,South China Agricultural University,Guangzhou 510642)
出处
《植物遗传资源学报》
北大核心
2025年第8期1597-1605,I0001-I0008,共17页
Journal of Plant Genetic Resources
基金
广州市科技计划(2024A04J5656)
广东省省级科技计划(2022B0202160015)
广东省乡村振兴战略专项资金-种业振兴(2022-NPY-00-027)。
关键词
苦瓜亚种
遗传图谱
果实相关性状
QTL
bitter gourd subspecies
genetic map
fruit-related traits
QTL
