摘要
目的:研究复方扶芳藤合剂(compound fufangteng mixture,CFM)对环磷酰胺(cyclophosphamide,CTX)诱导的免疫抑制小鼠作为治疗性给药还是预防性给药的最佳方式。方法:将BALB/c小鼠随机分为空白组(Blank),CTX+CFM高、中、低剂量治疗组(CTX+CFM-1、2、3),CFM+CTX高、中、低剂量预防组(CFM+CTX-1、2、3),每组5只。通过腹腔注射给予Blank组生理盐水(0.02 mL/g),CTX+CFM-1、2、3通过腹腔注射给予70 mg/kg环磷酰胺,每日1次,连续3天,自第4天起,3组分别灌胃15、7.5、3.75 g/kg CFM,连续10天;CFM+CTX-1、2、3灌胃15、7.5、3.75 g/kg CFM,连续10天,自第11天起,通过腹腔注射给予70 mg/kg环磷酰胺,每日1次,连续3天。观察小鼠状态,监测小鼠体质量,检测末次小鼠血常规、胸脾指数、T淋巴细胞比例。结果:CFM+CTX预防组和CTX+CFM治疗组在改善CTX诱导的小鼠体质量、脾脏指数、T淋巴细胞比例方面无明显差异(P>0.05),而CTX+CFM治疗组在改善免疫抑制小鼠的胸腺指数和淋巴细胞数量方面优于CFM+CTX预防组(P<0.05)。结论:CFM作为治疗性给药,更有利于改善CTX诱导的免疫抑制小鼠的免疫状态。
Objective:To investigate the best method of compound Fufangteng mixture(CFM)given through therapeutic or preventive administration to the mice with cyclophosphamide(CTX)induced immunosuppression.Methods:BALB/c mice were randomized into the blank group(Blank),the treatment groups of high,moderate and low doses of CTX+CFM(CTX+CFM-1,2,3),and the prevention groups of high,moderate and low doses of CFM+CTX(CFM+CTX-1,2,3),five mice in each group.Physiological saline was administered to Blank group through peritoneal injection(0.02 mL/g),CTX+CFM-1,2 and 3 were given 70 mg/kg CTX by intraperitoneal injection,once each day,for three days in a row,from the 4th day,the three groups were given 15 g/kg,7.5 g/kg and 3.75 g/kg CFM,for ten consecutive days.CFM+CTX-1,2 and 3 were drenched with 15 g/kg,7.5 g/kg and 3.75 g/kg CFM for ten days continuously,three groups accepted intraperitoneal injection of 70 mg/kg CTX since the 11th day,once each day,for three days consecutively.To observe the state of the mice,to monitor the body weight,and to detect routine blood test,spleen index and the proportions of T lymphocytes in mice.Results:No significant difference has been found in the improvements of body weights,spleen indexes,and the proportions of T lymphocytes in mice with CTX-induced immunosuppression between the prevention groups of CFM+CTX and the treatment groups of CTX+CFM(P>0.05),while the treatment groups of CTX+CFM was obviously better than the prevention groups of CFM+CTX in improving thymus index and the lymphocytes count in immunosuppres-sion mice(P<0.05).Conclusion:CFM as preventive administration is more beneficial to improve the immune state of CTX-induced immunosuppressed mice.
作者
陈果
刘显
彭丽珊
李建明
吴展帅
肖健
CHEN Guo;LIU Xian;PENG Lishan;LI Jianming;WU Zhanshuai;XIAO Jian(Guangxi Key Laboratory of Translational Medicine for Treating High-Incidence Infectious Diseases with Integrative Medicine,Nanning 530200,China;Guangxi University of Chinese Medicine,Nanning 530200,China;Teaching Experiment and Training Center,Guangxi University of Chinese Medicine,Nanning 530001,China)
出处
《西部中医药》
2025年第7期12-17,共6页
Western Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81860780)
国家自然科学基金地区基金(82460863)
广西自然科学基金(2020GXNSFAA297162)
广西自然科学基金青年科学基金(2020GXNSFBA159064)
2018年广西中医药大学校级课题(2018QN028)
2018年广西博士科研启动金(2018BS015)。
关键词
复方扶芳藤合剂
免疫抑制
T淋巴细胞
compound Fufangteng mixture
immunosuppression
T lymphocytes
