摘要
目的:探讨胃癌组织中微小RNA-1297(miR-1297)、支链氨基酸转氨酶1(BCAT1)表达与病理参数、上皮-间充质转化(EMT)及预后的关系。方法:选取2017年1月至2019年9月在我院接受手术切除的胃癌患者150例(胃癌组)和同期胃肠息肉手术患者65例(对照组),采用实时荧光定量聚合酶链反应检测胃癌组织和胃肠息肉组织中miR-1297、BCAT1 mRNA和EMT标志物[波形蛋白(VIM)、蜗牛家族转录抑制因子1(SNAI1)、钙黏蛋白(E-cad)、N-钙黏蛋白(N-cad)]表达。通过在线数据库预测miR-1297与BCAT1的结合位点,Pearson相关分析胃癌组织中miR-1297、BCAT1 mRNA与EMT标志物表达的相关性;根据胃癌组织中miR-1297、BCAT1 mRNA表达均值分为高、低表达组,通过Kaplan-Meier法绘制不同miR-1297、BCAT1 mRNA表达胃癌患者生存曲线;多因素Cox回归分析miR-1297、BCAT1 mRNA表达与胃癌患者预后的关系。结果:与胃肠息肉组织比较,胃癌组织miR-1297、E-cad mRNA低表达,BCAT1 mRNA、VIM mRNA、SNAI1 mRNA、N-cad mRNA高表达(P<0.05)。miR-1297与BCAT1的3'-非翻译端4135-4142处存在结合位点。胃癌组织中miR-1297与BCAT1 mRNA、VIM mRNA、SNAI1 mRNA、N-cad mRNA表达呈负相关,与E-cad mRNA表达呈正相关(P<0.05);BCAT1 mRNA与VIM mRNA、SNAI1 mRNA、N-cad mRNA表达呈正相关,与E-cad mRNA表达呈负相关(P<0.05)。低分化、TNM分期Ⅲ期、有淋巴结转移的胃癌组织中miR-1297表达低于中高分化、TNM分期Ⅰ~Ⅱ期、无淋巴结转移组织,BCAT1 mRNA表达高于中高分化、TNM分期Ⅰ~Ⅱ期、无淋巴结转移组织(P<0.05)。150例胃癌患者3年总生存率61.33%(92/150)。高miR-1297表达患者3年总生存率高于低miR-1297表达患者,高BCAT1 mRNA表达患者3年总生存率低于低BCAT1 mRNA表达患者(P<0.05)。低分化、TNM分期Ⅲ期、淋巴结转移、BCAT1 mRNA≥2.38为胃癌患者死亡的独立危险因素,miR-1297≥1.33为独立保护因素(P<0.05)。结论:胃癌组织中miR-1297低表达,BCAT1 mRNA高表达,与不良病理特征、EMT和预后有关,可能成为评估胃癌患者EMT和预后的新型标志物。
Objective:To investigate the relationship between the expression of microRNA-1297(miR-1297)and branched-chain amino acid transaminase 1(BCAT1)in gastric cancer tissues with pathological parameters,epithelial-mesenchymal transition(EMT)and prognosis.Methods:A total of 150 gastric cancer patients who underwent surgical resection from January 2017 to September 2019(gastric cancer group)and 65 patients undergoing gastrointestinal polyp surgery during the same period(control group)were selected.The expression of miR-1297,BCAT1 mRNA,and EMT markers[vimentin(VIM),snail family transcriptional repressor 1(SNAI1),E-cadherin(E-cad),N-cadherin(N-cad)]in gastric cancer and gastrointestinal polyp tissues were detected using real-time quantitative polymerase chain reaction.Binding sites between miR-1297 and BCAT1 were predicted through an online database,and the correlation between the expression of miR-1297,BCAT1 mRNA,and EMT markers in gastric cancer tissues was analyzed using Pearson correlation analysis.Patients were grouped into high and low expression groups based on the mean expression levels of miR-1297 and BCAT1 mRNA,and Kaplan-Meier survival curves were plotted for patients with different miR-1297 and BCAT1 mRNA expression levels.Multivariate Cox regression analysis was used to explore the relationship between miR-1297,BCAT1 mRNA expression,and prognosis in gastric cancer patients.Results:Compared with gastrointestinal polyp tissues,miR-1297 and E-cad mRNA expression levels were lower in gastric cancer tissues,while BCAT1 mRNA,VIM mRNA,SNAI1 mRNA,and N-cad mRNA expression levels were higher(P<0.05).A binding site was identified at the 3′untranslated region(4135-4142)of BCAT1 for miR-1297.In gastric cancer tissues,miR-1297 expression was negatively correlated with BCAT1 mRNA,VIM mRNA,SNAI1 mRNA,and N-cad mRNA expression but positively correlated with E-cad mRNA expression(P<0.05).BCAT1 mRNA expression was positively correlated with VIM mRNA,SNAI1 mRNA,and N-cad mRNA but negatively correlated with E-cad mRNA(P<0.05).miR-1297 expression was lower and BCAT1 mRNA expression higher in poorly differentiated,TNM stage Ⅲ,and lymph node metastatic gastric cancer tissues compared to moderately and well-differentiated,TNM stage Ⅰ-Ⅱ,and non-metastatic tissues(P<0.05).The 3-year overall survival rate of 150 gastric cancer patients was 61.33%(92/150).Patients with high miR-1297 expression had higher 3-year overall survival rates than those with low miR-1297 expression,while patients with high BCAT1 mRNA expression had lower survival rates compared to those with low BCAT1 mRNA expression(P<0.05).Poor differentiation,TNM stage Ⅲ,lymph node metastasis,and BCAT1 mRNA≥2.38 were independent risk factors for mortality,while miR-1297≥1.33 was an independent protective factor(P<0.05).Conclusion:miR-1297 expression is low and BCAT1 mRNA expression is high in gastric cancer tissues,and both are associated with unfavorable pathological characteristics,EMT,and poor prognosis.These factors may serve as novel biomarkers for evaluating EMT and prognosis in gastric cancer patients.
作者
张霞
邓双年
杨晓鹃
ZHANG Xia;DENG Shuangnian;YANG Xiaojuan(Linfen People's Hospital,Shanxi Linfen 041000,China)
出处
《河北医学》
2025年第7期1145-1151,共7页
Hebei Medicine
基金
山西省医师协会医师临床科研项目,(编号:YSXH-QL2023XB002)。
关键词
胃癌
微小RNA-1297
支链氨基酸转氨酶1
病理参数
上皮-间充质转化
Gastric cancer
MicroRNA-1297
Branched-chain amino acid transaminase 1
Pathological parameters
Epithelial-mesenchymal transition
