摘要
目的:探讨温阳通痞消积方(WYTP)是否可通过调控线粒体凋亡途径促进小鼠肝癌细胞凋亡。方法:培养肝癌H22细胞,并建立H22肝癌移植瘤裸鼠模型,将裸鼠分为对照组、阳性药物组、温阳通痞消积方(WYTP)低剂量(WYTP-L)组、WYTP中剂量(WYTP-M)组和WYTP高剂量(WYTP-H)组;观察肿瘤生长情况并计算肿瘤抑制率;HE染色观察肿瘤组织形态;线粒体红色荧光探针分析线粒体膜电位(MMP)变化;TUNEL染色检测肿瘤组织细胞凋亡;Western blot检测细胞Cleaved-Caspase-9、Caspase-9、Caspase-3、Cleaved-Caspase-3、Cyt C、Bax、Bcl-2、PCNA蛋白表达水平。结果:各组裸鼠生存状态基本良好。与对照组比较,WYTP-L组、WYTP-M组和WYTP-H组和阳性药物组裸鼠肿瘤细胞排列疏松,可见不同程度核固缩和空泡现象,有细胞坏死现象,肿瘤质量、体积、线粒体膜电位、Bcl-2和PCNA蛋白表达水平降低,抑瘤率、肿瘤细胞凋亡数量、肿瘤组织细胞Cleaved-Caspase-9、Caspase-9、Caspase-3、Cleaved-Caspase-3、Cyt C和Bax蛋白表达水平升高(P<0.05);阳性药物组与WYTP-H组裸鼠上述指标均无统计学差异(P>0.05)。结论:WYTP通过调节线粒体细胞凋亡途径可促进肝癌小鼠细胞凋亡,并抑制细胞增殖。
Objective:To investigate whether the Wenyang Tongpi Xiaoji prescription(WYTP)can promote apoptosis of liver cancer mouse cells by regulating the mitochondrial apoptosis pathway.Methods:H22 liver cancer cells were cultured,and a nude mouse model of H22 liver cancer transplantation was established.The nude mice were assigned to a control group,a positive drug group,a low-dose WYTP(WYTP-L)group,a medium-dose WYTP(WYTP-M)group,and a high-dose WYTP(WYTP-H)group.Tumor growth was observed,and tumor inhibition rate was calculated.HE staining was used to observe the morphology of tumor tissues.Mitochondrial membrane potential(MMP)was analyzed by mitochondrial red fluorescent probe.TUNEL staining was performed to detect cell apoptosis in tumor tissues.Western blot was used to detect the protein expression levels of Cleaved-Caspase-9,Caspase-9,Caspase-3,Cleaved-Caspase-3,Cyt C,Bax,Bcl-2,and PCNA.Results:The survival status of nude mice in each group was basically good.Compared with the control group,the tumor cells of nude mice in the WYTP-L group,WYTP-M group,WYTP-H group,and positive drug group showed loose arrangement,varying degrees of nuclear condensation and vacuolization,and cell necrosis,the tumor mass,tumor volume,mitochondrial membrane potential,the protein expression levels of Bcl-2 and PCNA decreased,the tumor inhibition rate,number of apoptotic tumor cells,and expression levels of Cleaved-Caspase-9,Caspase-9,Caspase-3,Cleaved-Caspase-3,Cyt C,and Bax proteins in tumor tissue cells increased(P<0.05).There was no statistically prominent difference in the above indicators between the positive drug group and the WYTP-H group(P>0.05).Conclusion:WYTP can promote apoptosis of liver cancer mouse cells and inhibit cell proliferation by regulating the mitochondrial apoptosis pathway.
作者
杨春
向彩琼
覃鑫
邹丹
张军
郑新平
徐大志
YANG Chun;XIANG Caiqiong;QIN Xin(Jingzhou Traditional Chinese Medicine Hospital,Hubei Jingzhou 434000,China)
出处
《河北医学》
2025年第7期1092-1097,共6页
Hebei Medicine
基金
湖北省中医药科研立项项目,(编号:ZY2023F095)
荆州市科技局项目,(编号:2024HD168)。
关键词
肝癌细胞
温阳通痞消积方
线粒体凋亡途径
凋亡
线粒体膜电位
Hepatocellular carcinoma cells
Wenyang Tongpi Xiaoji prescription
Mitochondrial apoptosis pathway
Apoptosis
Mitochondrial membrane potential
