摘要
为揭示宁夏清水河地表水、沉积物及河岸带土壤3种生境中的真菌群落结构、多样性及其影响因素,2022年在清水河设置了22个采样点.采样时间涵盖平水期(5月)、丰水期(7月)和枯水期(12月),采用高通量测序技术,并结合环境因子检测与多元统计分析方法进行研究.结果显示:总体上地表水中的真菌α多样性低于沉积物,但群落结构对水期的响应更为敏感,而沉积物与河岸带土壤中的真菌群落相似性较高.优势门类包括壶菌门(Chytridiomycota)、子囊菌门(Ascomycota)、担子菌门(Basidiomycota),未明确鉴定属(Fungi_gen_Incertae_sedis)、镰刀菌属(Fusarium)为关键优势属.地表水的水温(WT)、pH值、碳酸氢根(HCO3-)和钙离子(Ca2+),沉积物的pH值、电导率(EC)、速效钾(AK)、总磷(TP)和间隙水的pH值、总溶解性固体(TDS)、氨氮(NH3-N),河岸带土壤的水溶态氟(Ws-F)、可交换性钙(Ex-Ca)、总氮(TN)、TP等对真菌群落影响显著.真菌指示类群的变化反映不同水期的环境特征:平水期表现为农业面源污染富集,丰水期指示有机污染物迁移,枯水期则与难降解有机物的积累以及高盐碱环境相关.
To investigate the fungal community structure,diversity,and influencing factors in three habitats(surface water,sediment,and riparian soil)of the Qingshui River in Ningxia,22 sampling sites were established along the river in 2022.Sampling was conducted during the three hydrological periods:the normal period(May),the wet season(July),and the dry season(December).High-throughput sequencing technology was employed in conjunction with environmental factor measurements and multivariate statistical analyses.The results showed that theα-diversity of fungi in surface water was lower than that in sediment.However,the community structure in surface water was more sensitive to hydrological periods.In contrast,the fungal communities in sediment and riparian soil showed higher similarity.The dominant phyla were Chytridiomycota,Ascomycota,and Basidiomycota,and Fungi_gen_Incertae_sedis and Fusarium were identified as the key dominant genera.Water temperature(WT),pH value,bicarbonate(HCO3−)and calcium(Ca2+)in surface water,pH value,conductivity(EC),available potassium(AK),total phosphorus(TP)in sediments,pH value,total dissolved solids(TDS),ammonia nitrogen(NH3-N)in interstitial water,water-soluble fluorine(Ws-F),exchangeable calcium(Ex-Ca),total nitrogen(TN),TP in riparian soil had significant effects on fungal community.The changes of fungal indicator groups reflected the environmental characteristics of different hydrological periods:the accumulation of agricultural non-point source pollution in the normal period,the migration of organic pollutants in the wet season,and the accumulation of refractory organics and high saline-alkali environment in the dry season.
作者
赵增锋
邱小琮
尹娟
杨强强
徐程
侯洪飞
王凯
倪成
ZHAO Zeng-feng;QIU Xiao-cong;YIN Juan;YANG Qiang-qiang;XU Cheng;HOU Hong-fei;WANG Kai;NI Cheng(School of Civil and Hydraulic Engineering,Ningxia University,Yinchuan 750021,China;School of Life Science,Ningxia University,Yinchuan 750021,China)
出处
《中国环境科学》
北大核心
2025年第8期4563-4572,共10页
China Environmental Science
基金
宁夏自然科学基金重点项目(2023AAC02026)
宁夏高等学校一流学科建设(水利工程)项目(NXYLXK2021A03)。
关键词
清水河
真菌
群落结构
多样性
影响因素
Qingshui River
fungi
community structure
diversity
influencing factor
