摘要
探究薏苡素在薏苡(Coix lacryma-jobi L.)幼苗期、分蘖期、拔节期、孕穗期、抽穗开花期、成熟期6个生长时期中根、茎、叶、种子中薏苡素的含量水平,并明确薏苡素在薏苡植株中的合成积累规律。采集薏苡不同生长期根、茎、叶、种子样品,使用超声辅助提取法提取,利用液相色谱与质谱联用仪(UPLC-MS/MS)检测薏苡素含量。结果表明,薏苡不同生长期各部位薏苡素含量存在显著差异,根中薏苡素含量最高[(661.06±52.41)mg/kg],其次为叶[(80.73±2.31)mg/kg]、种子[(14.56±0.29)mg/kg]和茎[(9.80±0.44)mg/kg],各部位薏苡素含量与株高、根长、鲜重呈负相关。不同部位对比发现,孕穗期薏苡的薏苡素产量达最大值[(112.47±9.20)mg/株],其次为抽穗开花期[(51.42±7.96)mg/株]。单株薏苡素产量与株高、根长、鲜重呈正相关。由此可见,孕穗期根中薏苡素积累量最大。
The content levels of coixol in roots,stems,leaves and seeds of coix(Coix lacryma-jobi L.)during six growth periods,namely,seedling,tillering,jointing,booting,blooming,and maturity were investigated,and the synthesis and accumulation pattern of coixol in coix plants were clarified.Samples of roots,stems,leaves and seeds of coix were collected during different growth periods,extracted by the ultrasound-assisted extraction method,and the content of coixol was detected by liquid chromatography coupled with mass spectrometry(UPLC-MS/MS).The results showed that there were significant differences in the content of coixol in different parts of coix at different growth stages,and the highest content of coixol was found in roots[(661.06±52.41)mg/kg],followed by leaves[(80.73±2.31)mg/kg],seeds[(14.56±0.29)mg/kg]and stems[(9.80±0.44)mg/kg].The content of coixol in each part was negatively correlated with plant height,root length,and fresh weight.Comparison of different parts of the plant revealed that coix obtained the greatest coixol yield at the booting stage[(112.47±9.20)mg/plant],followed by the blooming stage[(51.42±7.96)mg/plant].Coixol yield per plant was positively correlated with plant height,root length and fresh weight.It could be seen that the maximum accumulation of coixol was in the roots at the booting stage.
作者
王耀
李福敏
王敏
杨天明
孙大利
WANG Yao;LI Fu-min;WANG Min;YANG Tian-ming;SUN Da-li(School of Public Health,Guizhou Medical University,Guiyang 561113,China)
出处
《湖北农业科学》
2025年第7期114-119,共6页
Hubei Agricultural Sciences
基金
贵州省科技支撑项目(黔科合支撑[2022]151)
中央支持地方高校改革发展资金项目(黔教技[2023]067号)。
