摘要
目的采用四维数据非依赖性采集(4-Dimensional Data-Independent Acquisition,4D-DIA)蛋白质组学技术,探究二至丸延缓衰老的作用机制。方法提取大鼠血清蛋白质,进行质谱检测及DIA数据分析。以log2 Foldchange≥1且P<0.05为条件筛选差异表达蛋白,并对其进行GO和KEGG通路富集分析。结果经血清蛋白组学分析,共鉴定蛋白7866个。与自然衰老模型组比较,二至丸高、中、低剂量组差异表达蛋白分别为206、187、172个;通过GO和KEGG通路富集分析发现,差异表达蛋白共同富集在移植物抗宿主疾病、Th1和Th2细胞分化、移植排斥等通路。结论二至丸可能通过调节自然衰老大鼠蛋白表达,调控移植物抗宿主疾病、Th1和Th2细胞分化、移植排斥等通路,影响其免疫功能,进而发挥延缓衰老作用。
Objective To investigate the mechanism of action of Er’zhi Pill(二至丸,EZP)in delaying aging using 4-dimensional data-independent acquisition(4D-DIA)proteomics technology.Methods Serum proteins were extracted from rats and analyzed via mass spectrometry and DIA data processing.Differentially expressed proteins(DEPs)were screened with the criteria of log2 Foldchange≥1 and P<0.05.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed on the identified DEPs.Results A total of 7,866 proteins were identified through serum proteomics analysis.Compared with the natural aging model group,the high/medium/low-dose EP groups exhibited 206,187,and 172 DEPs,respectively.GO and KEGG pathway enrichment analysis revealed that these DEPs were co-enriched in pathways such as graft-versus-host disease,Th1 and Th2 cell differentiation,and transplant rejection.Conclusion EZP may exert anti-aging effects by regulating protein expression in naturally aging rats,modulating pathways related to graft-versus-host disease,Th1 and Th2 cell differentiation,and transplant rejection,thereby influencing immune function.
作者
刘敏
朱雨墨
谢静仪
洪伟
单思
LIU Min;ZHU Yumo;XIE Jingyi;HONG Wei;SHAN Si(Research Center for Differentiation and Development of CM Basic Theory of Jiangxi University of Chinese Medicine,Nanchang 330004,China;Jiangxi Province Key Laboratory of TCM Etiopathogenisis,Nanchang 330004,China)
出处
《时珍国医国药》
北大核心
2025年第15期2995-3000,共6页
JOURNAL OF LI-SHIZHEN TRADITIONAL CHINESE MEDICINE
基金
江西省自然科学基金面上项目(20232BAB206147)
江西中医药大学博士科研启动基金课题(2019WBZR010)
江西中医药大学校级科技创新团队发展计划项目(CXTD22007)。
关键词
二至丸
衰老
蛋白组学
通路
作用机制
Er’zhi Pill(二至丸)
Aging
Proteomics
Pathway
Mechanism of action
