摘要
为了评估本团队研发的复方羟氯扎胺混悬液对西门塔尔肉牛自然感染肝片吸虫(Fasciola hepatica)和胃肠道线虫(GINs)的临床药效,并确定其最佳临床推荐剂量,为其临床应用提供参考依据。本试验将75头经虫卵筛选呈阳性的西门塔尔肉牛随机分为5个组,每组15头,分别为复方羟氯扎胺混悬液低剂量组[0.125 mL/(kg·bw)]、中剂量组[0.250 mL/(kg·bw)]、高剂量组[0.500 mL/(kg·bw)]、对照药物组[Levafas diamond,0.250 mL/(kg·bw)]和空白对照组,分别在给药后第0、28天,采集血液,进行血液生理生化指标测定;在给药后第0、7、14、21、28天,采集每头牛的新鲜粪便,测定肝片吸虫和GINs的每克粪便虫卵数(EPG),计算精计驱虫率和虫卵转阴率。结果显示,给药后第0、28天,各给药组与空白对照组的血液生理生化指标无显著差异(P>0.05)。在给药后第28天,与空白对照组相比,低剂量组、中剂量组、高剂量组和对照药物组肝片吸虫和GINs的EPG均显著减少(P<0.05);在给药后第14天,各给药组精计驱虫率和虫卵转阴率均达到最大值。结果表明,复方羟氯扎胺混悬液对西门塔尔肉牛肝片吸虫和GINs具有显著的驱杀效果,推荐复方羟氯扎胺混悬液的临床应用剂量为0.250 mL/(kg·bw)。
This study aimed to evaluate the clinical efficacy of a self-developed compound oxyclozanide suspension against Fasciola hepatica and gastrointestinal nematodes(GINs)in naturally infected Simmental beef cattle,and to determine the optimal clinical dosage for practical application.A total of 75 Simmental beef cattle confirmed positive for helminth eggs were randomly divided into five groups(15 animals per group):Low-dose[0.125 mL/(kg·bw)],Medium-dose[0.250 mL/(kg·bw)],and Highdose[0.500 mL/(kg·bw)]compound oxyclozanide suspension groups,Control drug group[Levafas diamond,0.250 mL/(kg·bw)],and Blank control group.Blood samples were collected on days 0 and 28 post-treatment to analyze hematological and biochemical indexes.Fresh fecal samples were collected from each animal on days 0,7,14,21,and 28 post-treatment to determine egg per gram(EPG)counting of F.hepatica and GINs,and to calculate the precise deworming rate and egg-negative conversion rate.The results showed no significant differences in hematological or biochemical indexes between any treatment groups and the Blank control group on days 0 and 28(P>0.05).By day 28 post-treatment,all treated groups(Low-dose,Medium-dose,High-dose,and Control drug group)showed significantly lower EPG values compared with the Blank control group(P<0.05).On day 14,the precise deworming rate and egg-negative conversion rate reached their peak in all treated groups.The findings demonstrate that the compound oxyclozanide suspension exhibits significant anthelmintic activity against F.hepatica and GINs in Simmental beef cattle.The recommended clinical dosage for practical use is 0.250 mL/(kg·bw).
作者
张志锦
商子璇
周绪正
白玉彬
徐晶
戴钰茵
李海權
李杰航
朱阵
张继瑜
ZHANG Zhijin;SHANG Zixuan;ZHOU Xuzheng;BAI Yubin;XU Jing;DAI Yuyin;LI Haiquan;LI Jiehang;ZHU Zhen;ZHANG Jiyu(College of Life Sciences and Food Engineering,Hebei University of Engineering,Handan 056038,China;Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Key Laboratory of Veterinary Pharmaceutics Discovery,Ministry of Agriculture and Rural Affairs,Gansu Provincial Key Laboratory of New Veterinary Drugs,Lanzhou 730050,China)
出处
《中国兽医杂志》
北大核心
2025年第8期21-30,共10页
Chinese Journal of Veterinary Medicine
基金
中国农业科学院创新工程项目(25-LZIHPS-05)
国家肉牛牦牛产业技术体系(CARS-37)。
关键词
复方羟氯扎胺混悬液
西门塔尔肉牛
肝片吸虫
胃肠道线虫
药效学
compound oxyclozanide suspension
Simmental beef cattle
Fasciola hepatica
gastrointestinal nematodes
pharmacodynamics
