摘要
目的探讨食物蛋白诱导过敏性直肠结肠炎(food protein-induced allergic proctitis,FPIAP)患儿临床特征及巨噬细胞移动抑制因子(mobility inhibitory factor,MIF)与疾病的关系。方法收集入组健康体检婴幼儿(n=7)及FPIAP(n=20)患儿年龄、性别、临床症状、血生化指标等一般临床资料;以“food protein-induced allergic proctocolitis”、“FPIAP”、“food allergy”、“allergy”、“cow milk protein allergy”、“CMPA”为关键词,检索基因表达综合数据库,通过数据库深挖掘技术分析出过敏相关差异基因;进一步利用Luminex检测技术,检测对照组和FPIAP组患儿血清中MIF、IL-1β、IL-4、IL-6、IL-8、IL-10及TNF-α的表达情况;最后通过免疫组织荧光技术观察MIF蛋白在结肠黏膜的表达情况。结果两组在性别、发病年龄、居住地等资料比较,差异无统计学意义(P>0.05);临床症状中腹泻、便血、厌食症状比较,差异有统计学意义(P<0.05),而哭闹、皮疹症状差异无统计学意义(P>0.05)。FPIAP患儿血化验指标中EOS、PLT、LDA、CK-MB水平较对照组升高,差异有统计学意义(P<0.05);HGB水平则较对照组降低,差异有统计学意义(P<0.05);而WBC、ALT、AST、CK、CREA水平差异无统计学意义(P>0.05)。数据库深挖掘技术发现MIF基因过敏性哮喘组明显上调。Luminex技术发现FPIAP患儿血清中MIF、IL-1β、IL-4、IL-6、IL-8及TNF-α的表达水平升高,差异有统计学意义(P<0.001),尤其以MIF升高较为明显;而IL-10的表达水平差异无统计学意义(P>0.05)。免疫组织荧光染色验证了MIF蛋白在FPIAP患儿结肠黏膜的表达明显增多。结论MIF是参与FPIAP疾病进展的重要因子,可结合临床症状、血生化指标共同提高该病诊断的准确性和简便性,有作为靶点治疗该疾病的可能性。
Objective To investigate the clinical characteristics of food protein-induced allergic proctitis(FPIAP)and the relationship between macrophage mobility inhibitory factor(MIF)and FPIAP.Methods The clinical data such as age,gender,clinical symptoms,and blood biochemical indicators of healthy control(n=7)and FPIAP(n=20)were collected.Keywords including"food protein-induced allergic proctocolitis","FPIAP","food allergy","allergy","cow milk protein allergy",and"CMPA"were used to search the GEO database.Data was further analyzed.Then,we use Luminex to check the serum expression of MIF,IL-1β,IL-4,IL-6,IL-8,IL-10 and TNF-α.Finally,immunofluorescence technology was used to observe the expression of MIF in the colonic mucosa.Results There was no significant difference in sex,age and place of residence between the two groups(P>0.05).The clinical symptoms of diarrhea,hematochezia and anorexia had statistical difference(P<0.05),while crying and rash had no statistical difference(P>0.05).The EOS,PLT,LDA and CK-MB levels of FPIAP patients were significantly higher than those of the control group(P<0.05),while HGB was significantly lower than that of the control group(P<0.05).There was no difference in WBC,ALT,AST,CK,CREA(P>0.05).The gene level of MIF was significantly up-regulated in allergic asthma(AA)patients.Luminex showed the serum levels of MIF,IL-1β,IL-4,IL-6,IL-8 and TNF-αin FPIAP were increased(P<0.001),particularly with a more noticeable increase in MIF.Whereas the IL-10 level showed no significant difference(P>0.05).Immunofluorescence staining confirmed that protein MIF in FPIAP colonic mucosa was significantly increased.Conclusions MIF is an important factor involved in the progression of FPIAP,which can be combined with clinical symptoms and blood biochemical indicators to improve the accuracy and simplicity of FPIAP diagnosis,and has the potential to be a target for treating the FPIAP.
作者
薛福敏
侯江珊
孙波
郑丽娟
郭亚琼
王跃生
于静
XUE Fumin;HOU Jiangshan;SUN Bo;ZHENG Lijuan;GUO Yaqiong;WANG Yuesheng;YU Jing(Henan Key Laboratory of Children's Genetics and Metabolic Diseases,Children's Hospital Affiliated to Zhengzhou University,Zhengzhou 450000;Department of Gastroenterology,Children's Hospital Affiliated to Zhengzhou University,China)
出处
《胃肠病学和肝病学杂志》
2025年第7期1013-1017,1022,共6页
Chinese Journal of Gastroenterology and Hepatology
基金
河南省科技发展计划项目(242102310056,242102310070)
河南省医学科技攻关计划联合共建项目(LHGJ20230571,LHGJ20210626)。
