摘要
以矿区原生植物狼尾草(Pennisetum alopecuroides)和极小短小杆菌(Curtobacterium pusillum)为研究对象,通过盆栽试验,在不同Cd污染水平土壤中接种极小短小杆菌,研究该菌种对狼尾草生长特性的影响,并考察狼尾草对土壤中Cd的耐受性。结果表明,接种极小短小杆菌能显著提高狼尾草的抗氧化酶活性,以及非酶抗氧化剂、渗透调节物质和金属硫蛋白(MTs)含量,可减轻Cd胁迫造成的氧化损害,改善狼尾草的生长。在6.00 mg/kg的Cd污染水平下,接种极小短小杆菌的狼尾草中过氧化物酶(POD)活性和谷胱甘肽(GSH)含量比未接种的对照组分别高167.44%和89.20%,可溶性糖(SS)和MTs含量分别增加27.81%和25.32%,根长和株高显著提高59.84%和47.23%(P<0.05)。
In this study,C.pusillum and the native plant P.alopecuroides in the mining area were taken as the research objects.Through pot experiments,C.pusillum was inoculated in soils with different levels of Cd pollution to study its effects on the growth characteristics of P.alopecuroides and to investigate the tolerance of P.alopecuroides to Cd in soil.The results showed that inoculation with C.pusillum could significantly enhance the antioxidant enzyme activity of P.alopecuroides,increase the content of non-enzymatic antioxidants,osmotic regulators and metallothionein(MTs)in P.alopecuroides,alleviate the oxidative damage caused by Cd stress and improve the growth of P.alopecuroides.When the Cd content was 6.00 mg/kg,the peroxidase(POD)activity and glutathione(GSH)content in P.alopecuroides inoculated with C.pusillum were 167.44% and 89.20% higher,respectively,than those in the uninoculated control group.The contents of soluble sugar(SS)and MTs increased by 27.81% and 25.32%,respectively.The root length and plant height were significantly increased by 59.84%and 47.23%(P<0.05),respectively.
作者
丁丹
陈诚
单士锋
郑刘根
DING Dan;CHEN Cheng;SHAN Shifeng;ZHENG Liugen(No.321 Unit,Bureau of Geology and Mineral Exploration of Anhui Provice,Tongling,Anhui 244000,China;School of Resources and Environmental Engineering,Anhui University,Hefei,Anhui 230601,China;Anhui Laboratory of Mining Ecological Restoration Engineering,Hefei,Anhui 230601,China)
出处
《环境监测管理与技术》
北大核心
2025年第4期79-83,99,共6页
The Administration and Technique of Environmental Monitoring
基金
国家自然科学基金资助项目(42072201)
安徽省高校协同创新基金资助项目(GXXT-2021-017)
铜陵市环保督察重点基金资助项目(232107)。
关键词
极小短小杆菌
狼尾草
镉
耐受性
植物修复
Curtobacterium pusillum
Pennisetum alopecuroides
Cadmium
Tolerance
Phytoremediation
