摘要
【目的】为明确厚朴“发汗”过程中微生物群落与厚朴品质的相关性,探究关键微生物对厚朴中厚朴酚及和厚朴酚积累的影响机制。【方法】采用传统培养分离技术结合高效液相色谱分析方法,系统分离和筛选厚朴“发汗”过程中的关键功能菌株。通过分子生物学技术对目标菌株进行鉴定,并构建系统发育树分析其进化地位和亲缘关系。采用单因素试验结合BoxBehnken响应面法对菌株HP3“发汗”工艺参数进行优化。【结果】从“发汗”厚朴样本中分离纯化出48株菌,经筛选发现有9株能够显著提升厚朴酚与和厚朴酚产量的菌株,其中HP3菌株效果最佳,经鉴定HP3菌株为贝莱斯芽胞杆菌(Bacillus velezensis);对HP3菌株的“发汗”工艺参数优化发现,其最佳“发汗”工艺参数为发汗温度31.84℃、发汗时间1.9 d、水煮时间6.37 min、干燥温度80℃,在此条件下,厚朴酚与和厚朴酚总量高达5.149%,较未“发汗”样品提升62.67%。【结论】从厚朴“发汗”过程中分离鉴定出一株菌株HP3,该菌株可显著提高厚朴“发汗”过程中厚朴酚与和厚朴酚的总含量。此外,通过响应面优化试验确定了菌株HP3的最佳“发汗”工艺条件。研究揭示了厚朴“发汗”过程中与品质密切相关的细菌类群,为厚朴药材的微生物资源开发提供了重要依据。
【Objective】To clarify the correlation between the microbial colonies and the quality of Magnolia officinalis during the process of“sweating”,and to investigate the regulatory mechanisms of key microbial species on the biosynthesis and accumulation of magnolol and honokiol in M.officinalis.【Method】Traditional culture separation and HPLC were systematically employed to isolate and screen functionally critical microbial strains during the“sweating”process of M.officinalis.The target strains were identified using molecular biological techniques,and phylogenetic trees were constructed to analyze their evolutionary status and genetic relationships.The process parameters of“sweating”of strain HP3 were optimized by using single-factor experiments combined with the Box-Behnken response surface method.【Result】Forty-eight strains were isolated and purified from the“sweating”M.officinalis samples.Subsequent screening revealed 9 strains that significantly increased the production of magnolol and honokiol.Among these,strain HP3 presented the most pronounced efficacy and was identified as Bacillus velezensis.The optimization of the“sweating”process parameters for strain HP3 revealed the following optimal conditions:Sweating temperature 31.84℃,sweating time 1.9 d,boiling time 6.37 min and drying temperature 80℃.Under these conditions,the total amount of magnolol and honokiol was as high as 5.149%, an increase of 62.67% compared with the non-sweated samples.【Conclusion】 A strain HP3 was isolated and identified from the “sweating” process of M. officinalis, which demonstrated remarkable capability in enhancing the total content of magnolol and honokiol. Furthermore, the optimal sweating process parameters for strain HP3 were determined through response surface optimization experiments. The study has revealed the bacterial communities closely related to the quality of M. officinalis during its “sweating” process, providing an important basis for the development of microbial resources of M. officinalis medicinal materials.
作者
张茹
李一鸣
张桐溪
孙占斌
任清
潘寒姁
ZHANG Ru;LI Yi-ming;ZHANG Tong-xi;SUN Zhan-bin;REN Qing;PAN Han-xu(School of Light Industry Science and Engineering,Beijing Technology and Business University,Beijing 100048)
出处
《生物技术通报》
北大核心
2025年第8期322-334,共13页
Biotechnology Bulletin
关键词
厚朴
分离鉴定
厚朴酚
和厚朴酚
“发汗”
Magnolia officinalis
isolation and identification
magnolol
honokiol
“sweating”
