摘要
【目的】糖苷水解酶17(glycoside hydrolase 17, GH17)在植物、动物和真菌的多种生理及致病过程中发挥重要作用,对大麦条纹病菌PgGH17家族成员进行鉴定和分析,为后续深入研究GH17基因功能提供科学依据,同时为解析大麦条纹病菌的致病性基因功能和探究寄主-病原互动机制奠定坚实的理论基础。【方法】以大麦条纹病菌为研究对象,通过生物信息学方法,对基因家族进行鉴定及分析,经实时荧光定量PCR(RT-qPCR)监测大麦条纹病菌感染过程中各基因不同时间点的表达变化。【结果】PgGH17基因家族含有6个成员,氨基酸序列长度为305-648 aa,均为亲水性蛋白,等电点为5.06-8.66,脂肪指数为65.43-82.00,信号肽长度为0-22 aa,且大多定位在细胞外基质,只有1个基因具有跨膜结构。内含子数为1-3个,进化分析表明,该基因家族成员可聚成7个亚族。RT-q PCR结果表明,除PgGH17-3外,其他PgGH17基因在侵染过程中的表达水平基本呈现先上升后下降的趋势。【结论】从大麦条纹病菌中鉴定出6个GH17家族成员,除GH17-3外,其他GH17基因在侵染过程中表达均增强,说明其参与了病原菌的致病性。
【Objective】Glycoside hydrolase 17(GH17)plays a significant role in various physiological and pathogenic processes in plants,animals and fungi.The identification and preliminary analysis of the PgGH17 family members in the Pyrenophora graminea provide a scientific basis for further in-depth research on the function of GH17 genes,and concurrently lay a solid theoretical foundation for understanding the function of pathogenicity genes in the P.graminea and exploring the host-pathogen interaction mechanism.【Method】Using P.graminea as the research subject,we identified and analyzed gene families through bioinformatics approaches.Subsequently,real-time quantitative PCR(RT-qPCR)was employed to monitor the temporal expression patterns of these genes during the infection process.【Result】The PgGH17 gene family comprises six members,with amino acid sequence lengths ranging from 305 to 648 aa.All proteins are hydrophilic,exhibiting isoelectric points(pI)of 5.06‒8.66 and aliphatic indices of 65.43‒82.00.Signal peptide lengths vary from 0 to 22 aa,with most members localized to the extracellular matrix;only one gene harbors a transmembrane domain. Intron numbers range from 1 to 3 per gene. Phylogenetic analysis revealed that these family members cluster into seven distinct subclades. Quantitative reverse transcription PCR (RT-qPCR) results demonstrated that, with the exception of PgGH17-3, the expressions of other PgGH17 genes during infection generally present first an initial upregulation followed by subsequent downregulation.【Conclusion】Six GH17 family members are identified in P. graminea, and with the exception of GH17-3, all show upregulated expressions during infection, suggesting their functional involvement in the pathogenicity of this fungal pathogen.
作者
任睿斌
司二静
万广有
汪军成
姚立蓉
张宏
马小乐
李葆春
王化俊
孟亚雄
REN Rui-bin;SI Er-jing;WAN Guang-you;WANG Jun-cheng;YAO Li-rong;ZHANG Hong;MA Xiao-le;LI Bao-chun;WANG Hua-jun;MENG Ya-xiong(State Key Laboratory of Arid Habitat Crop Science,Gansu Provincial Key Laboratory of Crop Genetic Improvement and Germplasm Innova-tion,Lanzhou 730070;College of Agronomy,Gansu Agricultural University,Lanzhou 730070;College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070)
出处
《生物技术通报》
北大核心
2025年第8期146-154,共9页
Biotechnology Bulletin
基金
国家大麦青稞产业技术体系项目(CARS-05-03B-03)
国家自然科学基金项目(31960426,32160496)
甘肃省自然科学基金重点项目(24JRRA637)。
