摘要
目的采用两样本孟德尔随机化(MR)方法探讨干果摄入与结直肠癌的因果关系。方法利用干果摄入与结直肠癌的全基因组关联研究汇总数据进行两样本MR分析,并单独选择结肠癌和直肠癌数据进行验证。选取与干果摄入显著相关并相互独立的遗传变异作为工具变量,使用逆方差加权法、MR-Egger回归、加权中位数法、简单模式法、加权模式法作为主要分析方法,并进行异质性检验、多效性分析以及敏感性分析用于评价研究的可靠性。结果共纳入21个干果摄入相关的单核苷酸多态性(SNPs)作为工具变量。逆方差加权法分析显示,干果摄入与结直肠癌发生风险降低相关(OR=0.55,95%CI:0.32~0.96,P=3.53×10^(-2)),结肠癌与直肠癌的分析结果也一致。本研究结果不受多效性、异质性的影响,敏感性分析验证了结果的可靠性。结论增加干果摄入对结直肠癌具有潜在的预防价值。
Objective To investigate the causal relationship between dried fruit intake and colorectal cancer(CRC)by using a two-sample Mendelian randomization(MR)approach.Methods Two-sample MR analysis was conducted utilizing summary data from genome-wide association studies on dried fruit intake and colorectal cancer.Separate analyses were performed using data for colon cancer and rectal cancer for validation.Genetic variants significantly and independently associated with dried fruit intake were selected as instrumental variables.The inverse variance weighted method,MR-Egger regression,weighted median method,simple mode method,and weighted mode method were employed as the primary analytical approaches.Heterogeneity tests,pleiotropy analysis,and sensitivity analysis were conducted to evaluate the reliability of the study.Results A total of 21 single nucleotide polymorphisms(SNPs)associated with dried fruit intake were included as instrumental variables.The inverse variance weighted analysis revealed that increased dried fruit intake was associated with a reduced risk of colorectal cancer(OR=0.55,95%CI,0.32 to 0.96,P=3.53×10^(-2)),with consistent results observed for both colon cancer and rectal cancer.The findings of this study were not influenced by pleiotropy or heterogeneity,and the reliability of the results was validated by sensitivity analysis.Conclusion Increasing dried fruit intake has potential preventive value against colorectal cancer.
作者
金志超
戴虹
杨冉
JIN Zhichao;DAI Hong;YANG Ran(Department of Oncology,the Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing,Jiangsu,210029)
出处
《实用临床医药杂志》
2025年第13期39-43,49,共6页
Journal of Clinical Medicine in Practice
基金
国家自然科学基金资助项目(81904110)
江苏省科技厅青年基金资助项目(BK20191086)
江苏省中医院高峰学术人才培养工程(y2021rc46)
江苏省中医院院级课题(Y22005)。
关键词
干果摄入
结直肠癌
孟德尔随机化
全基因组关联分析
因果关系
疾病风险
逆方差加权法
加权中位数法
dried fruit intake
colorectal cancer
Mendelian randomization
genome-wide association analysis
causal relationship
disease risk
inverse variance weighted method
weighted median method
