摘要
目的研究X射线辐照淋巴细胞凋亡状况及红细胞辐照后不同保存期血液质量变化,以评估X射线的辐照效果及辐照红细胞临床使用的最佳时机。方法选取无偿献血者捐献的血液13份,每份先用4个试管各留取5 mL全血,分为d0、d7、d14、d21四组,在相应保存期提取淋巴细胞,分成淋巴细胞对照组与辐照组,辐照组以25 Gy的X射线辐照,用流式细胞术检测各组淋巴细胞凋亡率;剩余血液制备成去白悬浮红细胞,分为红细胞对照组及d0、d7、d14红细胞辐照组,各红细胞辐照组分别在相应的保存期用25 Gy的X射线进行辐照,检测辐照后第0、7、14天及相应保存期红细胞对照组的钾离子(K^(+))、游离血红蛋白(FHb)、三磷酸腺苷(ATP)和2,3-二磷酸甘油酸(2,3-DPG)的变化。结果X射线辐照可有效诱导淋巴细胞发生凋亡,凋亡率随全血保存时间延长而逐渐降低。d0、d7、d14淋巴细胞辐照组的淋巴细胞凋亡率均显著高于同期对照组(P<0.05),d21淋巴细胞辐照组淋巴细胞凋亡率与对照组无显著差异(P>0.05)。d0、d7红细胞辐照组K+含量在辐照后第7、14天,d14红细胞辐照组在辐照后第0、7、14天均高于相应的对照组,差异具有统计学意义(P<0.05),其余各组差异无统计学意义(P>0.05)。对于FHb含量,d7红细胞辐照组在辐照后第14天、d14红细胞辐照组在辐照后第7天和第14天均高于相应的对照组,差异具有统计学意义(P<0.05),其余各组差异无统计学意义(P>0.05)。d14红细胞辐照组ATP含量在辐照后第14天的含量低于对照组,差异具有统计学意义(P<0.05),其余各组差异无统计学意义(P>0.05)。各红细胞辐照组2,3-DPG含量与对照组比较差异均无统计学意义(P>0.05)。结论25 Gy X射线在14天保存期内对淋巴细胞的辐照效果显著,可致淋巴细胞凋亡。红细胞辐照时机对辐照后不同保存期的血液质量有所影响,特殊患者输注辐照红细胞应特别关注辐照时机及辐照后的保存时间。
Objective To investigate the apoptosis status of lymphocytes exposed to X-ray irradiation and evaluate changes in the quality of red blood cells(RBCs)at different preservation periods post-irradiation,thereby assessing the efficacy of X-ray irradiation and determining the optimal timing for clinical application of irradiated RBCs.Methods Thirteen blood samples donated by voluntary donors were selected.For each sample,5 mL of whole blood was collected into four test tubes and divided into four groups:day 0(d0),day 7(d7),day 14(d14)and day 21(d21).Lymphocytes were extracted during the corresponding preservation periods and further categorized into a lymphocyte control group and an irradiation group.The irradiation group was exposed to 25 Gy X-ray irradiation,and the apoptosis rate of lymphocytes in each group was measured using flow cytometry.The remaining blood was processed into leukoreduced suspended RBCs and divided into an RBCs control group and irradiation groups(d0,d7,and d14).Each irradiation group was exposed to 25 Gy X-rays during the respective storage periods.Changes in potassium(K^(+)),free hemoglobin(FHb),adenosine triphosphate(ATP),and 2,3-diphosphoglyceric acid(2,3-DPG)were detected on days 0,7,and 14 post-irradiation and during the corresponding preservation periods.Results X-ray irradiation effectively induced lymphocyte apoptosis,with the apoptosis rate gradually decreasing as the preservation time of whole blood increased.The apoptosis rates of lymphocytes in the d0,d7,and d14 irradiation groups were significantly higher than those in the control groups during the same periods(P<0.05).However,no significant difference in apoptosis rate was+observed between the d21 irradiation group and the control group(P>0.05).The K content in the d0 and d7 irradiation groups on days 7 and 14 post-irradiation,as well as in the d14 irradiation group on days 0,7,and 14 post-irradiations,was significantly higher than that in the corresponding control groups(P<0.05).No statistically significant differences were observed in the other groups(P>0.05).The FHb content in the d7 irradiation group on day 14 post-irradiation and in the d14 irradiation group on days 7 and 14 post-irradiation was significantly higher than that in the corresponding control groups(P<0.05).No statistically significant differences were observed in the other groups(P>0.05).The ATP content in the d14 irradiation group was significantly lower than that in the control group on day 14 post-irradiation(P<0.05).No statistically significant differences were observed in the other groups(P>0.05).There were no statistically significant differences in 2,3-DPG content between the irradiation groups and the control group(P>0.05).Conclusion Exposure to 25 Gy X-rays has a significant effect on lymphocytes within the first 14 days of preservation,inducing apoptosis.The timing of RBCs irradiation influences blood quality at different preservation periods post-irradiation.When administering irradiated RBCs to special patients,careful consideration should be given to the timing of irradiation and the preservation period post-irradiation.
作者
安立
刘群
王秀芝
刘艳
贡觉顿珠
赵爱平
梁雪峰
庄云龙
AN Li;LIU Qun;WANG Xiuzhi;LIU Yan;GONGJUE Dunzhu;ZHAO Aiping;LIANG Xuefeng;ZHUANG Yunlong(Shandong Blood Center,Jinan 250012)
出处
《临床输血与检验》
2025年第4期484-491,共8页
Journal of Clinical Transfusion and Laboratory Medicine
基金
山东省医药卫生科技发展计划项目(No.202202080955)资助。
关键词
X射线
辐照
淋巴细胞
凋亡
红细胞
血液质量
X-ray
Irradiation
Lymphocyte
Apoptosis
Red blood cell
Blood quality
