摘要
目的对桂枝-甘草药对单煎液和合煎液的入血成分进行分析比较,为桂枝-甘草药对的药效物质基础研究提供参考。方法通过UPLC-Q-TOF-MS/MS技术,根据提取离子流色谱图、质谱碎片裂解信息识别目标化合物,结合TCMSP、BATMAN-TCM以及知网等综合性数据库,筛选入血成分。对桂枝、甘草以及药对含药血清的化学成分进行分析。结果桂枝、甘草及其药对均含有丰富的化学成分。从桂枝、甘草及其药对含药血清中分别鉴定出103个、164个、143个化学成分。其中桂枝入血原型成分20个,代谢产物83个;甘草含药血清入血原型成分41个,潜在入血原型成分代谢产物123个;桂枝-甘草药对含药血清入血原型成分31个,代谢产物112个;主要入血成分包括黄酮类、酚类、苯丙素类及萜类。结论本研究对桂枝-甘草单煎液和合煎液入血成分进行了定性分析,明确了其入血成分的差异。为桂枝-甘草药对的药效物质基础和作用机制的进一步研究提供参考。
OBJECTIVE To analyze and compare the blood-entry components of single decoction and combined decoction of Cinnamomi Ramulus,Glycyrrhizae Radix et Rhizoma,and their medicinal pairs,so as to provide a reference for the basic research on the pharmacological substances.METHODS The target compounds were identified by UPLC-Q-TOF-MS/MS technique based on extracted ion flow chromatograms,mass spectrometry fragmentation cleavage information,and screened for haematological constituents by combining comprehensive databases such as TCMSP,BATMAN-TCM,and CNKI networks.The chemical composition of Cinnamomi Ramulus-Glycyrrhizae Radix et Rhizoma medicinal pair containing serum was analysed.RESULTS The Cinnamomi Ramulus,Glycyrrhizae Radix et Rhizoma,and their medicinal pairs were rich in chemical constituents.A total of 103,164,143 constituents were identified from the blood serum of Cinnamomi Ramulus,Glycyrrhizae Radix et Rhizoma,and their medicinal pairs,respectively.Cinnamomi Ramulus plasma samples containing 20 potential prototype bloodentry components,and 83 metabolites of potential prototype blood-entry components.Glycyrrhizae Radix et Rhizoma plasma samples containing 41 potential prototype blood-entry components,and 123 metabolites of potential prototype blood-entry components.Cinnamomi Ramulus-Glycyrrhizae Radix et Rhizoma medicinal pairs plasma samples containing 31 potential prototype blood-entry components,and 112 metabolites of potential prototype blood-entry components.Mainly blood-entry components including flavonoids,phenolics,phenylpropanoids,alkaloids,and other components.CONCLUSION In this study,the blood-entry components of Cinnamomi Ramulus-Glycyrrhizae Radix et Rhizoma single decoction and combined decoction are qualitatively analysed,and the differences in blood-entry components are clarified.It provides a reference for further studies on the pharmacological material basis and mechanism of action of Cinnamomi Ramulus-Glycyrrhizae Radix et Rhizoma medicinal pairs.
作者
王鹤
王智超
雷紫文
冯李乐
邹俊波
史亚军
栾飞
WANG He;WANG Zhichao;LEI Ziwen;FENG Lile;ZOU Junbo;SHI Yajun;LUAN Fei(School of Pharmacy,Shaanxi University of Chinese Medicine,Shaanxi Provincial Key Laboratory of Chinese Medicine Basic and New Chinese Medicine Research,Xianyang 712046,China)
出处
《中国现代应用药学》
北大核心
2025年第14期2407-2416,共10页
Chinese Journal of Modern Applied Pharmacy
基金
陕西省中医药管理局中医药科研项目(SZY-KJCYC-2025-JC-043)
陕西中医药大学科技创新人才体系建设计划项目(2024-CXTD-03)。
关键词
桂枝-甘草药对
化学成分
入血成分
UPLC-Q-TOF-MS/MS
Cinnamomi Ramulus-Glycyrrhizae Radix et Rhizoma medicinal pairs
chemical composition
blood-entry component
UPLC-Q-TOF-MS/MS
