摘要
目的观察手足口病应用PCR实时荧光定量技术检测的临床价值。方法选取2022年2月至2022年9月在漳平市总医院收治的手足口病198例患者,按照不同的检测方式分为观察组99例,予以PCR实时荧光定量检测技术,对照组99例,为PCR常规检测技术,分析两组对于肠道病毒分型的阳性检出率、特异性、灵敏性。结果观察组中,经检测发现肠道病毒CVA16呈阳性的病例数为71例,CVA6呈阳性的病例数为13例,未检测到EV71阳性病例,而EV通用型呈阳性的病例数达到98例。相较之下,对照组的检测结果显示,肠道病毒CVA16阳性病例为30例,CVA6阳性病例为3例,同样未检出EV71阳性病例,EV通用型阳性病例数为80例,除EV71型肠道病毒外,观察组在其他肠道病毒分型的阳性检出率方面均高于对照组(P<0.05);通过采用CVA16、肠道病毒通用及EV71特异性引物进行PCR扩增试验,结果显示两种检测方法在肠道病毒亚型鉴定方面具有高度一致性,证实其对HFMD病原体的检测特异性良好;采用10倍系列稀释法制备4.11×10^(7) CFU/ml的HFMD病原体混合样本进行灵敏度测试,比较分析表明两种检测方法均展现出优异的检测下限发现,观察组的检验最低限为4.11×10^(2) CFU/ml;对照组的检验最低限为4.11×10^(3) CFU/ml,与对照组相比,观察组的检测的灵敏度更高(P<0.05)。结论肠道病毒检测中予以PCR荧光定量检测技术具有快速检测、较高的灵敏性和特异性,对于手足口病的快速诊断提供有利的检验方式。
Objective To observe the clinical value of PCR real-time fluorescence quantitative technique in the detection of hand foot mouth disease.Methods A total of 198 patients with hand-foot-mouth disease who were admitted to Zhangping General Hospital from February 2022 to September 2022 were selected and divided into an observation group of 99 cases according to different detection methods,which was treated with PCR real-time fluorescence quantitative detection technology,and a control group of 99 cases,which was treated with PCR conventional detection technology.The positive detection rate,specificity and sensitivity of enterovirus typing in the two groups were analyzed.Results In the observation group,71 cases were found to be positive for enterovirus CVA16,13 cases were found to be positive for CVA6,no EV71 positive cases were detected,and 98 cases were positive for EV universal type.In contrast,the test results of the control group showed that there were 30 positive cases of enterovirus CVA16 and 3 positive cases of CVA6.No positive cases of EV71 were detected either.The number of positive cases of EV71 universal type was 80.Except for EV71 enterovirus,The positive detection rate of other enterovirus subtypes in the observation group was higher than that in the control group(P<0.05).Through PCR amplification experiments using CVA16,universal enterovirus and EV71-specific primers,the results showed that the two detection methods had a high degree of consistency in the identification of enterovirus subtypes,confirming their good specificity in the detection of HFMD pathogens.The mixed samples of HFMD pathogens with a concentration of 4.11×10^(7) CFU/ml were prepared by the 10-fold series dilution method for sensitivity testing.Comparative analysis indicated that both detection methods exhibited excellent lower detection limits.It was found that the lowest detection limit of the observation group was 4.11×10^(2) CFU/ml.The minimum test limit of the control group was 4.11×10^(3) CFU/ml.Compared with the control group,the detection sensitivity of the observation group was higher(P<0.05).Conclusions PCR fluorescence quantitative detection technology in enterovirus detection has rapid detection,high sensitivity and specificity,and provides a favorable test method for the rapid diagnosis of hand foot mouth disease.
作者
付珊珊
陈景平
张彦锋
FU Shanshan;CHEN Jingping;ZHANG Yanfeng(Laboratory Testing Section,Zhangping Center for Disease Control and Prevention,Longyan,Fujian 364400,China)
出处
《中国医药指南》
2025年第21期109-112,共4页
Guide of China Medicine
关键词
手足口病
肠道病毒71型
柯萨奇病毒A组16型
PCR荧光定量检测技术
Hand foot mouth disease
Enterovirus 71
Coxsackievirus group A type 16
PCR fluorescence quantitative detection technology
