摘要
该研究建立了一种超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法(UHPLC-Q-Orbitrap-MS)同时检测预制菜中多种防腐剂和甜味剂的分析方法。样品经乙腈水溶液振荡、离心、净化、过滤后上机,经Waters ACQUITY UPLC HSS T3色谱柱(2.1mm×100mm,1.8μm)分离,以流动相A(甲醇-5mmol/L乙酸铵溶液)和流动相B(乙腈)进行梯度洗脱,在负离子模式下进行检测。结果表明,防腐剂和甜味剂在各自线性浓度范围内,线性关系良好,相关系数为0.9945~1.0000,检出限为0.002~0.10mg/kg,定量限为0.008~0.35mg/kg,低、中、高三个浓度水平加标回收率为70.3%~95.4%,相对标准偏差(RSD)为3.1%~13.8%,该方法可用于预制菜中多种防腐剂和甜味剂的快速检测。
This study developed an ultra high performance liquid chromatography-quadrupole/electrostatic field orbital trap high-resolution mass spectrometry(UHPLC-Q-Orbitrap-MS)analysis method for simultaneous detection of multiple preservatives and sweeteners in premade dishes.The sample was subjected to oscillation with acetonitrile aqueous solution,centrifugation,purification,and filtration before being loaded onto the machine,it was separated by Waters ACQUITY UPLC HSS T3(2.1 mm×100 mm,1.8μm)chromatographic column,gradient elution was performed using mobile phase A(methanol-5mmol/L ammonium acetate solution)and mobile phase B(acetonitrile),and the detection was performed in negative ion mode.The results showed that preservatives and sweeteners had a good linear relationship within their respective linear concentration ranges,with correlation coefficients ranging from 0.9945~1.0000,detection limit ranging from 0.002~0.10 mg/kg,and quantification limit ranging from 0.008~0.35 mg/kg.The spiked recovery rates of low,medium,high three concentration levels ranged from 70.3%~95.4%,and the relative standard deviation(RSD)range was 3.1%~13.8%.This method can be used for rapid detection of multiple preservatives and sweeteners in premade dishes.
作者
杨春梅
李兴玉
陈涛
黄静
马倩
吴雪莉
李敏婕
周晓群
孙涵
YANG Chunmei;LI Xingyu;CHEN Tao;HUANG Jing;MA Qian;WU Xueli;LI Minjie;ZHOU Xiaoqun;SUN Han(Chengdu Product Quality Supervision and Inspection Institute,Chengdu 610000,China;Chengdu Product Quality Inspection and Research Institute Co.,Ltd.,Chengdu 610000,China;Sichuan Food and Fermentation Industry Research&Design Institute Co.,Ltd.,Chengdu 611130,China;Sichuan Product Quality Supervision,Inspection and Testing Institute,Chengdu 610100,China)
出处
《食品与发酵科技》
2025年第4期117-123,共7页
Food and Fermentation Science & Technology
基金
四川省科技厅“基于流通环节的短保预制菜关键安全指标在线高效检测技术研究与运用(面上项目)”(2023YFN0059)。
关键词
预制菜
防腐剂
甜味剂
超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法
premade dishes
preservative
sweetener
ultra high performance liquid chromatography-quadrupole/electrostatic field orbital trap high-resolution mass spectrometry
