摘要
[Objectives]To study the network pharmacology-based anticancer effect of Ardisiacrispin B for colon cancer(CRC).[Methods]The chemical structure and molecular properties of Ardisiacrispin B were assessed via the PubChem resource,while the putative target genes of Ardisiacrispin B were predicted using the PharmMapper Database.Moreover,Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway and Gene Ontology(GO)enrichment analyses were conducted via the WebGestalt platform.Finally,a drug-target-pathway network was built via Cytoscape to show the visual representation.[Results]Ardisiacrispin B exhibited exceptional druggability with 25 putative targets.Analyses conducted using KEGG,GO,and network methods showed that these target genes were related with inflammatory responses,cancer,and varoius other biological functions.On the basis of these findings,we further screened the correlative biotargets of Ardisiacrispin B in relation to CRC,and explored the anticancer effects of Ardisiacrispin B for the treatment of CRC through CCK8 analysis and colony formation assay.Our results confirmed that Ardisiacrispin B exhibited anti-CRC properties,and suggested 11 candidate targets of Ardisiacrispin B in the treatment of CRC.[Conclusions]Ardisiacrispin B has been demonstrated to target multiple proteins/genes and pathways,thereby forming a network that displays systematic pharmacological activities.Moreover,it has potential therapeutic value in tumor treatment,specifically in promoting the proliferation of CRC cells.
基金
Supported by Medical Scientific Research Foundation of Guangdong Province of China(A2022479)
University-Hospital Joint Fund Project of Guangzhou University of Traditional Chinese Medicine(GZYZS2024U08).
