摘要
肌球蛋白重链(Myosin heavy chain)参与鱼类肌纤维肥大和增生过程,在肌肉生长和收缩过程中扮演重要角色。为筛选出与生长性状相关的单核苷酸多态性(Single nucleotide polymorphisms,SNPs)位点,采用PCR扩增技术获得海水经济养殖鱼类——杉虎杂交斑(棕点石斑鱼Epinephelus fuscoguttatus♀×清水石斑鱼E.polyphekadion♂)的肌球蛋白重链基因序列全长,并基于相关性分析筛选与生长性状相关的SNPs位点。结果表明,杉虎杂交斑肌球蛋白重链基因序列全长为12129 bp,包含36个外显子和35个内含子,可编码1934个氨基酸。该基因包含4种类型的蛋白结构域:Myosin_N、MYSc、IQ和Myosin_tail_1。经比对分析发现,该基因的核苷酸序列及蛋白结构域在鲈形目、鲀形目和鲽形目鱼类中均表现出较高的保守性。在肌球蛋白重链基因中检测到62个SNPs位点,其中8个符合Hardy-Weinberg平衡。有12个SNPs位点位于外显子区域,且均与体质量、全长等多个生长表型性状显著相关(p<0.05)。在这12个SNPs位点中仅有g5417 C>G为错义突变,导致丙氨酸变成甘氨酸;其余位点均为同义突变。这些与生长相关的SNPs位点可作为杉虎杂交斑生长分子标记开发的候选位点。
Myosin heavy chain(MYH)participates in hypertrophy and hyperplasia of muscle fibres in fish and plays an important role in muscle growth and contraction.In order to screen single nucleotide polymorphism(SNP)sites associated with growth traits,we applied the PCR amplification method to obtain the nucleotide sequence of MYH in the cultured marine fish,the hybrid grouper(Epinephelus fuscoguttatus♀×E.polyphekadion♂),and then used the correlation analysis to detect the SNPs related to its growth traits.The results show that the sequence length of the MYH was 12129 bp,including 36 exons and 35 introns,encoding 1934 amino acids.Four typical domains in MYH were detected,namely Myosin_N,MYSc,IQ and Myosin_tail_1.Comparative analysis reveals that high conservation in nucleotide sequence and protein domain of MYH was manifested in Perciformes,Tetraodontiformes and Pleuronectiformes species.Sixty-two SNP loci were found in the MYH.Eight SNP loci were in Hardy-Weinberg equilibrium state.Twelve SNP loci were located on exons,significantly correlated with growth traits,such as body mass and total length.Among the 12 SNP sites,only g5417 C>G was a missense mutation,resulting in the transformation from alanine to glycine.Other loci were synonymous mutations.These SNP sites related to growth provide candidate sites for screening the growth-related molecular markers in the hybrid grouper.
作者
曹柳
马军
卢艳
武雅彤
王钧
钟立静
苏珊珊
黄海
CAO Liu;MA Jun;LU Yan;WU Yatong;WANG Jun;ZHONG Lijing;SU Shanshan;HUANG Hai(Yazhou Bay Innovation Institute of Hainan Tropical Ocean University,Sanya 572022,China;Hainan Key Laboratory for Conservation and Utilization of Tropical Marine Fishery Resources,Sanya 572022,China;Key Laboratory of Utilization and Conservation for Tropical Marine Bioresources of Ministry of Education,Sanya 572022,China;College of Fisheries and Life Sciences,Hainan Tropical Ocean University,Sanya 572022,China)
出处
《南方水产科学》
北大核心
2025年第4期171-184,共14页
South China Fisheries Science
基金
海南热带海洋学院崖州湾创新研究院青年项目(2022CXYQNXM06)
海南省重大科技计划(ZDKJ2021017)
国家自然科学基金地区科学基金项目(32160861)
海南热带海洋学院崖州湾创新研究院重大科技计划项目(2022CXYZD001)。
关键词
杉虎杂交斑
肌球蛋白重链基因
生长性状
单核苷酸多态性位点
Epinephelus fuscoguttatus♀×E.polyphekadion♂
Myosin heavy chain gene
Growth traits
Single nucleotide polymorphism
