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190份大白菜自交系的遗传多样性分析及育种应用

Genetic diversity analysis and breeding application of 190 high inbred lines of Chinese cabbage
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摘要 为分析来源于26份亲本的190份大白菜高代自交系后代的主要表型性状与遗传多样性及其在育种上的应用潜力,对供试大白菜材料的27个主要表型性状进行变异分析、相关性分析,基于27对InDel标记进行遗传背景多样性分析。结果显示,27份大白菜主要性状的变异系数在10.10%~90.36%,变异丰富且多个表型性状具有高度相关性;选用的27对InDel标记的多态性信息含量变幅在0.01~0.37,经过分析190份大白菜高代自交系遗传相似系数在0.57~1.00,平均值为0.65,遗传变幅较大,基于InDel标记和群体结构均能将供试大白菜材料分为3个亚群,其中来源于16东郊引B和开蔬七号的后代分离较大。综上所述,选用的190份大白菜自交系表型性状变异范围大、遗传多样性丰富,可为配置不同性状优异材料提供材料基础,为配置性状优异材料提供研究基础。 In order to analyze the major phenotypic traits and genetic diversity of 190 high-generation inbred lines derived from 26 parental lines,and evaluate their potential for breeding applications,this study conducted variation and correlation analysis on 27 major phenotypic traits of the test materials,along with genetic diversity analysis based on 27 pairs In-Del markers.The results showed that the variation coefficient of the 27 phenotypic traits ranged from 10.10%to 90.36%,indicating substantial variability,with significant correlations observed among multiple traits.The InDel marker analysis revealed that the polymorphism information content ranged from 0.01 to 0.37,and the genetic similarity coefficient among the 190 high-generation inbred lines varied between 0.57 and 1.00,with an average of 0.65,suggesting considerable genetic variation within the population.Based on InDel markers and population structure analysis,the test materials could be divided into three subgroups,with the progeny derived from 16-Autumn introduced from eastern suburbs B and Kaishu No.7 exhibiting greater segregation.In conclusion,the 190 Chinese cabbage inbred lines used in this study exhibit broad phenotypic variation and rich genetic diversity,providing valuable germplasm resources and theroetical support for parental selection and the development of superior trait combinations in Chinese cabbage breeding.
作者 张文静 冯健起 苏贺楠 王培云 马骥 丁聪 魏小春 张晓伟 原玉香 ZHANG Wenjing;FENG Jianqi;SU Henan;WANG Peiyun;MA Ji;DING Cong;WEI Xiaochun;ZHANG Xiaowei;YUAN Yuxiang(Vegetable Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China;Kaifeng Academy of Agriculture and Forestry Sciences,Kaifeng 475004,Henan,China;Kaifeng Seed Industry Laboratory,Kaifeng 475004,Henan,China)
出处 《中国瓜菜》 北大核心 2025年第8期26-36,共11页 China Cucurbits And Vegetables
基金 中原科技创新领军人才(244200510041) 河南省农业良种联合攻关(2022010504) 国家大宗蔬菜产业技术体系(CARS-23-G15) 河南省农业科学院优秀创新团队(2024TD06) 中原学者工作站(ZYGZZ2024124) 白菜萝卜新品种选育与示范(254400510004)。
关键词 大白菜 表型性状 遗传多样性 INDEL标记 Chinese cabbage Phenotypic trait Genetic diversity InDel marker
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